| The resonance light scattering(RLS) methods have the advantages of conveninent operation and high sensitivity, and were perfectly applied to the determination of biomacromolecules and drugs.This paper selects a series of probes to detect drugs and DNA by the RLS technique.The study as follow:A new method for the detection of calf thymus DNA was proposed based on the enhanced resonance light scattering(RLS) signals of DNA in the presence of procyanidin and cetylpyridinium bromide dihydrate(CPB). This method has already reached the nanogram level. Under the experimental conditions, the RLS intensity of DNA at 291 nm was greatly enhanced by procyanidin–CPB at p H 7.0. There was a good linear relationship between the enhanced RLS intensity(RLS(35)I) and DNA concentration of 0.0084~3.36 μg·m L–1, and the regression equation was RLS(35)I = 6.70 + 70.72 c(c, μg·m L–1). The limit of detection(LOD) was 2.27 ng·m L–1. Three synthetic DNA samples were measured. The recovery was 102.3%~107.1% and RSD was 1.4%~4.1%.Two new ternary systems to detecting DNA based on aplinetin/morin in the presence of hexadecyl trimethyl ammonium bromide(CTAB) by resonance light scattering(RLS) was described. In p H 7.4, the RLS signal of DNA was remarkably increased due to the interaction between aplinetin/morin–CTAB and DNA. Good linear relationship betweenRLS(35)I and the DNA concentration for aplinetin/morin–CTAB– DNA was found. The detection limits(S/N = 3) were 2.71 ng·m L-1 and 2.41 ng·m L-1 respectively. The recoveries were 99.6%~102.6% and 96.6%~100.5% respectively.The DNA was determined sensitively using puerarin–Au NPs and quercetin –Au NPs as probes respectively. DNA can be bound to the puerarin–Au NPs or quercetin–Au NPs due to electrostatic interaction and aggregates, which results in a srong enhancement of the RLS intensity. The linear ranges were 0.0081~4.0 μg·m L–1 for quercetin–Au NPs–DNA and 0.0068~3.4 μg·m L–1 for puerarin–Au NPs–DNA. Three kinds of samples were studied with satisfactory results. The recoveries were 98.8%~101.7% and 98.7%~101.8% respectively.A novel RLS technique for detecting eugenol was established using gold nanorods(Au NRs) as probes which were synthesized. The weak RLS intensity of eugenol was obviously enhanced by the use of Au NRs. All of the results from the UV spectrum, RLS and SEM expressed that a new complex of Au NRs–eugenol was formed. The assembly of Au NRs–eugenol was achieved through a coordination bond between eugenol and Au NRs. The linear range was 0.043~10.60 μg·m L–1,the regression equation was RLS(35)I = 92.8 + 70.4 c(c,μg·m L–1),and LOD was 7.28 ng·m L-1. The recovery was 99.7%~104.2% and RSD(n = 5) was 0.81%~1.19% in three synthetic samples. The method was successfully uesed to analyse eugenol in curry powder samples.A sensitive RLS assay of aplinetin was developed based on aplinetin modified Au NRs. In p H 7.4 Tris–HCl buffer solution, the combination of aplinetin and Au NRs by electrostatic interaction resulted in the aggregation of aplinetin–Au NRs which showed high RLS intensity. Under optimum conditions, the magnitude of enhanced RLS intensity was proportional to the concentration of aplinetin over the range 0.027~3.24 μg·m L–1, with a detection limit 1.79 ng·m L–1. This developed RLS assay was successfully applied to the determinationn of aplinetin in three synthesized samples. The recovery was 95.1%~103.7%, RSD was 0.28%~3.9%(n = 5).A new RLS method for quercetin determination has been developed. In p H 8.0, quercetin reacted with Au NPs form a quercetin–Au NPs complex due to electrostatic interaction that resulted in considerable RLS characterized with a maximum peak at 467 nm. It is found that the enhanced RLS intensity was proportional to the concentration of quercetin in range of 0.0604~4.53 μg·m L–1 with the detection limit of 1.49 ng·m L–1. The proposed method possessed the advantages of sensitivity, straightforward and rapidity. The recovery was 96.7%~102.8% and RSD was 0.7%~1.5% in three synthetic samples. |
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