The Application Of FISH And Q-PCR Technology In The In-situ Biological Sample Analysis

Fluorescence in situ hybridization(FISH),microbial pure cultivating can be avoid, and it is clearly and conveniently observed from the sample for the quantity of cells and space positioning,and that through the different oligonucleotides probe,meanwhile on the cell level has a qualitative and quantitative analysis and space positioning identification for the different kind of microbial. Q-PCR include of the relative quantification and absolute quantification. The advantage of Q-PCR skill is that method simple, high specificity,time-consuming etc, and be widely used in microbial communities structure dynamic analysis for environmental sample. Obtain a fast and accurate analysis methods of FISH and Q- PCR technology about the biological metallurgy microbial species and the number of of sample preparation in the process of uranium deposits.Principal results are as follows:(1)TheAcidithiobacillus ferrooxidans are advantage species in K-JCK-22 samples which from 11-3 mining area in 512 uranium deposit in Xinjiang;(2)In terms of microbial count,compared with the MPN method, Fluorescence in situ hybridization and Q-PCR fast and accurate(3)Using RFLP method, fluorescence quantitative PCR method, fluorescence in situ hybridization technology research The proportion of Acidithiobacillus ferrooxidans,thiobacillus thiooxidans and Leptospirillum ferrooxidansin K-JCK-22samples:10.2:3.59:1,9.83:4.77:1,9.56:4.28:1.(4) FISH and the Q-PCR technique can be applied to the main species in the biological leaching uranium ore system for rapid detection of qualitative and quantitative;(5)Get a set of mature fluorescence in situ hybridization technique for acid samples of uranium ore and the pretreatment method of real-time fluorescent quantitative PCR technology.