Mixed-mode Hydrophilic/Reverse Packing Materials:Preparation And Its Properties For Chromatographic Separation

Chromatographic stationary phase is the core of high performance liquid chromatography(HPLC) system. Thereinto, development of novel stationary phases with high separation efficiency and excellent selectivity has always been the focus point of study.Mixed-mode stationary phases have been proved to be with the potential merits of high selectivity, remarkably high loading capacity and high separation efficiency. Among them,mixed-mode reversed-phase liquid chromatography(RPLC)/hydrophilic interaction chromatography(HILIC) stationary phases can be used for simultaneous separation of both hydrophilic and hydrophobic compounds, enlarging the scopes of application.This paper is divided into three chapters.Chapter One: A brief overview and classification of the mixed-mode stationary phases are illustrated.Chapter Two: A novel imidazolium embedded C8 mixed-mode stationary phase SIL-MPS-VOL was designed, synthesized and further characterized by Fourier transform infrared spectrometry and thermogravimetric analysis. The new stationary phase exhibited typical RPLC/HILIC retention mechanisms and a series of hydrophobic and hydrophilic test samples were successfully separated via the developed SIL-MPS-VOL stationary phase.Moreover, the developed stationary phase was further used to detect illegal addition of melamine to the powdered infant formula and polar components of secondary metabolites of Trichoderma, indicating a wide application potential.Chapter Three: A novel imidazolium-embedded iodoacetamide mixed-mode stationary phase Sil-VIM-IAA was prepared and further characterized by Fourier transform infrared spectrometry, elemental analysis, and thermogravimetric analysis, The retention of phenols and anilines exhibited “U” shaped curves, indicating the typical RPLC and HILIC retention mechanisms. Nucleosides/nucleic acid bases and isomers were used to evaluate the chromatographic performance of the developed Sil-VIM-IAA stationary phase and the results were further compared with that via the commercially available Globalsil TM APS column.Moreover, the developed stationary phase was further used to simultaneously separate of both hydrophilic and hydrophobic compounds, indicating a wide application potential.