Synthesis And Characterization Of Imidazolium Ionic Liquid Chromatographic Stationary Phase

High performance liquid chromatography (HPLC) is one of the most common means for separation and analysis.Stationary phase is the core of the whole HPLC system, so research on stationary phase is very important. In this paper, two kinds of liquid chromatographic stationary phase 2-ethyl-imidazole and 1-vinylimidazole ionic liquids were bonded with activated silica gel, and then their chromatographic performances were studied. The results are as follows:1.2-ethyl-imidazole chromatographic stationary phase was prepared using activated silica matrix,3-chloropropyl trimethoxysilane as a coupling agent,2-ethyl imidazole as a functional group, and trimethylchlorosilane as an end-capping reagent.After column packed, column dead time was tested by NaNO3, and the theoretical plate number was calculated by benzene. The separation experiments for aromatic compounds and flavonoids were made. We obtained a good separation efficiency and achieved baseline separation.2.Using the reaction between active silica gel Si-OH and KH-570,1-vinylimidazole was bonded on the surface of the modified silica gel via the double bond of KH-570,with BPO as an initiator and trimethylchlorosilane as a end-capping reagent. Finally we got 1-vinylimidazole liquid chromatography stationary phase.After column packed, column dead time was tested by NaNO3,and the theoretical plate number was calculated by benzene. KH-570 side chain structure effectively mask the unreacted silanols.The 1-vinylimidazole chromatographic packing showed outstanding performance in the analysis of aromatic compounds and flavonoids.And it was the first time that we attempt to separate protein by ionic liquid chromatographic packing.