Active Ingredients Extraction Study From Natural Products With MIP-Flash Chromatography

It has been a challenge to extract highly purified active ingredients from natural products due to their low content, complex matrix etc. In this study, molecularly imprinted polymers(MIPs) and Flash chromatography were combined to tackle this problem. And solanesol and L-quebrachitol with relatively high purity were successfully extracted from tobacco leaves and waste latex water respectively in this method.Solanesol MIPs(SMIPs) were prepared by suspension polymerization. After the recipes optimization, it was confirmed as followings, methyl methylacrylate(monomer), solanesol(template), ethyleneglycol dimethacrylate(crosslinker), azobisisobutyronitrile(initiator), polyvingakohol(surfactant), ultrapure water, chloroform(porogenic agent), and the ratio of template, monomer and crosslinker(T: M: C) was determined as 1: 6: 30. And the size range of SMIPs was 250~350 μm based on scanning electron microscope(SEM) results. Besides the imprinting factor was determined as 3.9 with solid phase extraction. After those polymers were packed in Flash chromatography, the binding capacity of SMIPs-Flash column to solanesol was calculated as 107.3 μmol/g. And 370.8 mg of solanesol(>97%)was separated from tobacco leaves(14.7 g).L-quebrachitol MIPs(QMIPs) were prepared by reverse phase suspension polymerization. After the recipes optimization, it was confirmed as followings, acrylamide and 4-vinyl benzene boric acid(comonomers), L-quebrachitol(template), N, N’-methylene-bis-(acrylamide)(crosslinker), potassium persulfate and azobisvaleronitrile(initiators), span-60(surfactant), ultrapure water and ethanol(porogenic agent), liquid paraffin, and the ratio of T: M:C was determined as 1: 2: 7. Then these spHerical polymers were packed in Flash chromatography, and the binding capacity of QMIPs-Flash column to L-quebrachitol was calculated as 45 mg/g. And 285.1 mg of L-quebrachitol(>94%) was purified from waste latex water(18.1 g).In this paper, MIPs with high selectivity were prepared and applied as Flash chromatography stationary phase. After the optimization of chromatographic condition, a chromatographic method aims at highly purified active ingredients extraction from natural products was developed.