Preparation Of Antibacterial/Antiviral Cyclodextrin Inclusion Complexes And Their Applications On Paper

With the rapid development of economy, the environment is becoming worse and worse. Pathogenic microbial is seriously threat to people’s health. Paper products are necessary in our daily life, so a lot of products are required to possess antibacterial and antiviral. However, the paper products with antibacterial/antiviral properties currently have only antibacterial /antiviral agent. Therefore, it is important to prepare synergistic antibacterial with two kinds of antibacterial agent or both antibacterial and antiviral paper products.Cyclodextrin(CDs) has the special structure with hydrophilic ektexine and hydrophobic cavity, which can form host-guest complex with appropriate molecular of molecules. In this paper, β-cyclodextrin grafting guanidine antimicrobial agent(β-CD-G) was prepared by grafted guanidine antimicrobial agent(PHGC) onto β-cyclodextrin using epichlorohydrin(ECH) as crosslinking agent. Grinding method was used for the formation of inclusion complexes β-CD-G-Cip and β-CD-G-AT between β-CD-G and Cip/AT.β-CD-G was synthesized by grafting PHGC to β-CD using ECH as a crosslinking agent. Molecular structure and antibacterial properties was characterized by fourier transform infrared spectroscopy(FTIR), proton nuclear magnetic resonance spectrometry(1H-NMR) and minimal inhibitory concentrations method(MIC), respectively. The optimal conditions and grafting efficiency were revealed by independent parameters:molar mass ratio,the dose of ECH and reaction temperature. The optimal condition for the synthesis of β-CD-G: n(ECH):n(β-CD)=3, n(β-CD):n(PHGC)=1:1.5, reaction temperature 60 ℃, at which the grafting efficiency and MIC was 19.3% and 250 ppm, respectively.Grinding method was used for the formation of inclusion complexes between β-CD-G and ciprofloxacin(Cip). The optimal grinding conditions of inclusion complexes: grinding time 30 min, n(Cip):n(β-CD)=2:1, m(H2O):m(β-CD-G)=1:1, at which, the loading amount of Cip in inclusion complexes was 19.8 mg/g β-CD-G. The release rate of amount of Cip from inclusion complexes increased almost linearly in the first 100 min, the released rate tended to be slow and reached equilibrium after 240 min, at which the cumulative release was about 85%. The antibacterial paper was prepared by dipping paper using the solution of β-CD-G-Cip and drying naturally. The antibacterial effect of antibacterial paper was measured using shake flack method and inhibition zone method, with increased of β-CD-G-Cip, the antibacterial effect of paper sheets increased significantly. When the adding amount of β-CD-G-Cip was 6%, the antibacterial rate of paper sheets was higher than 99%, the inhibition zone was 28.4 mm.Grinding method was used for the formation of inclusion complexes between β-CD-G and amantadine(AT). n(AT):n(β-CD)=2:1 was determined by job continuous ariation method. The optimal grinding conditions of inclusion complexes: grinding time 30 min, n(AT):n(β-CD)=2:1, m(H2O):m(β-CD-G)=1:1, at which, the loading amount of AT in inclusion complexes was 15.1 mg/g β-CD-G. The release rate of amount of AT from inclusion complexes increased almost linearly in the first 140 min, the released rate tended to be slow and reached equilibrium after 300 min, at which the cumulative release was about 86%. The antiviral paper was prepared by dipping paper using the solution of β-CD-G-AT and drying naturally. With increased of β-CD-G-AT, the antiviral effect of paper sheets increased significantly. When the adding amount of β-CD-G-AT was 10%, the antiviral rate of paper sheets was higher than 100%.