| Mesoporous silica nanoparticles with smaller particle size and high specific surface area, large pore volume, adjustable pore size, the surface can be chemically modified and good biological affinity and stability, and excellent properties, is widely used in the biomedical field, has been extended to drug delivery system to control is potential carriers of various drugs. At present it has been widely applied in the delivery of anticancer drugs, biological molecules, peptides,nucleotides, antibiotics, insoluble drug research. Which as a vaccine vector and adjuvant is one of a relatively new research field. The ideal vaccine adjuvants need to have safe and effective, targeted and economic characteristics, and nano porous silica nanoparticles is more in line with the requirements of vaccine adjuvant. Therefore, the nano porous silicon is one of the novel adjuvant materials.In this study, using the conventional sol-gel method synthesis of mesoporous silica nano-particles through a transmission electron microscope, the mesoporous silica particles are spherical, the particle size at about 400 nm, a substantially uniform distribution, obvious mesoporous structure, pore size 2-3 nm, characteristic of mesoporous materials is obvious.Re-use of genetic engineering technology to FMDV structural protein gene connected to the carrier, and then introduced into E. coli competent cells, prokaryotic expression system and purified FMDV capsid protein, obtained after digestion Vp0, Vp3, Vp1 three parts in the assembly buffer assembly to give type O FMD virus-like particles, particle size analysis indicated that the particle size 20-100 nm.The mesoporous silica nano-particle solution with FMD virus-like particle solution in accordance with a certain proportion of simple mixing, adsorption after a period of time, centrifugation, and then resuspended in PBS immunized animals. The experimental set-up mesoporous silica nanoparticles and two experimental groups with different contents of FMD virus-like particles, and set up a traditional vaccines Freund’s complete adjuvant and mouth disease virus-like particles as a control group, by contrast to evaluate the mesoporous nano immune effect of silica to FMD virus-like particles nano vaccines.Vaccine Evaluation mainly through the humoral immune response and cellular immune responses.This experiment using lymphocyte proliferation assay and lymphocyte factor content IFN-gama to evaluate effect of guinea pig immune cells. Enzyme linked immunosorbent assay(ELISA) was used to determination of type O FMDV specific antibody levels in response to humoral immune effect in guinea pigs.It was found that found that mesoporous silica nano vaccine and FMD virus-like particles can cause immune guinea pigs and humoral immune response cells, particularly humoral immune response may be due to the slow release properties of mesoporous silica FMD virus-like particle of good cause high antibody levels of immunized animals, the long duration. But compared with Freund’s adjuvant and traditional FMD virus-like particle vaccine in combination, as a vaccine adjuvant mesoporous silicon antibody levels or slightly lower number.From the Experimental results of virus protection, MSN + VLPs of nano vaccine immunized animals have a certain effect, the two control groups because there is no poison attack nor onset, PBS group and MSN group attack after the onset of all poison, Freund’s adjuvant + VLPs group and MSN + VLPs2 effect equivalent protection group, MSN + VLPs1 protective effect is slightly weaker, indicating that there is a positive correlation relations between protective efficacy and antigen dose effect. |
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