Synthesis And Application Research Of Pyrazoline And Isoxazoline Derivstives

This thesis can be divided into three sections.Chapter one: synthesis of the pyrazoline compounds and their application in org anic synthesis, drug discovery, reaction catalysis, electrochemistry and other fields.Sp ecifically describes the pyrazoline compounds excellent performance when used as a fluorescent material. Pyrazoline compounds having a high fluorescence quantum yield,high selectivity and outstanding stability; wherein the metal ions as a fluorescent pr obe, pyrazoline compounds have received increasing attention. Anthracene is a polyc yclic aromatic compound in a typical representative of this compound is characterize d by high fluorescence intensity, as the most common aromatic ring Anthracene ring is widely used in the design of a fluorescent acceptor. Based on the above ideas,designed and synthesized a novel pyrazoline fluorescent probes P1, P2.Chapter two: designed and synthesized two novel pyrazoline fluorescent probes P1 and P2. For the new probe compounds, its structure through 1H NMR, 13 C NMR,IR, HRMS characterization; testing its performance by fluorescent, we found that th e fluorescent probe P1 can fastly, efficiently identify Cu2 + and it has lower detectio n line. The probe is more suitable for real-time analysis in biological samples, the r esult shows that the probe can be applied in living cells to detect Cu2+ in PK-15 liv e-cell microscopic imaging experiments, cytotoxicity assays prove that the probe com pound to normal cells no significant cell toxicity for probe compound P2, we initiall y measured the UV absorption spectrum of the compound and fluorescence spectra t itration. The results show that the probe P2 can also quickly and effectively identify Cu2+.Chapter three: overview of the research progress in synthesis and application of isoxazoline compounds. Isoxazoline compounds can generally be two types of synth etic route: cyanide oxides and olefins [3+2] cycloaddition and α,β-unsaturated aldehy des / ketones with hydroxylamine reaction. Isoxazoline compounds are an important class of containing N, O,five-member heterocyclic compounds, due to the special st ructure in organic synthesis, drug discovery, reaction catalysis, electrochemistry and other fields,so they have a widely attention. In order to obtain the desired products,we choose diphenyl ether, chloride, aromatic aldehydes, hydroxylamine hydrochloride and other raw materials to synthesis of the new isoxazoline compounds. For novel compounds, their structures conducted by 1H NMR, 13 C NMR, IR and HRMS. Thepurpose of the experiment was to study the corresponding bioactive of the singl e and double Isoxazolines compounds. But the final result of the reaction was a littl e different from we expected.When the aromatic aldehydes containing electron-withdr awing groups,they can not give the desired products, so this experiment only got the novel compounds,we can not do the comparison of biological activity.