| Mesoporous silica nanoparticles(MSNs) has large specific surface, adjustable pore size, particle size, large pore volume, and excellent biological, blood, cells and tissue compatibility. At the same time, it is easy to realize surface functionalization to achieve targeted aim, due to abundant active silicon hydroxyl groups on the MSNs surface. MSNs is of great significance to design and manufacture of materials for third and the fourth generation of drug delivery system. Antibody protein G250(carbonic anhydrase IX, CAIX) almost has no expression in normal cells, but can excessive expression in cancer cells, its could be a very good tumor cell markers candidate.In this thesis, First MSNs was prepared.In the post-prepared, firstly mercaptopropyl was attached to the external surface through chemical bounds of the MSNs through condensation reaction. Secondly, it formed disulfide bond, through the knocked redox reaction, between the grafted thiol groups mentioned above and 2,2’-Dithiodipyridine molecules in solution. Thirdly, again, the G250 was tethered successfully onto the outside surface of the MSNs through reaction change of disulfide bound between the innate thiol group of G250 antibody protein molecule and disulfide bond gained aforementioned on the external surface of MSNs. All ths procedure and the products were verified by the combination of SEM, TEM, N2 isotherm, small angle XRD, EDX, FI-IR, Raman, TGA, 29Si-MAS NMR etc. skills. To verifity further the possibility of MSNs served as drug delivery system, the following some works including, at first, the behaviour of the selected modle drug molecules doxorubicin(DOX) loaded by MSNs-SH and the DOX drug molecules released from DOX@MSNs-SH were investgated; the Second, the cytotoxicity of MSNs, DOX@MSNs, MSNs-SH, DOX@MSNs-SH to hela cells was tested through CCK-8 assay. Results showed that the G250@MSNs is a potential of the third or the fourth generation drug delivery system candidate. |
PDF Full Text Request