| WIC biologics based on natural biologics stevia residue chrysanthemum as the main ingredient is refined. Developers of a variety of bioactive WIC biologics conducted extensive research and found that it has anti-oxidation, anti-allergy variety of biological activities, Xi Yin Ci found WIC biologics have strong antioxidant activity, and in 1998, the Japanese Society for food union has just released the world’s first paper on stevia(WIC biologics) antioxidant papers. WIC biologics strong antioxidant effects of main substances are polyphenols, which is WIC biologics strong antioxidant active ingredients Isolation and structure identification results derived, Xi Ci Yin Research based on the study made by Yu Hui. Prevention of hypertension, diabetes, obesity, heart disease and other disease is WIC natural biologics possess efficacy.Probiotic Lactobacillus casei is one of the Ministry of Health published in 2001, and its source is very wide, not only fresh vegetables and fruits themselves contain a small amount of probiotics and lactic acid fermentation products are also a good source of probiotics. Hot research at home and abroad probiotic Lactobacillus is on R & D thermophilic Lactobacillus, Bifidobacterium and Bulgaria Streptococcus strain, meat products, belonging to the probiotic Lactobacillus casei main application is in ferment agent products. Fermented lactic acid bacteria have strong vitality, inoculation less high content of viable, easy to use and the advantages of long storage period is applied to the food industry. To Lactobacillus casei better practical application, researchers have studied a lot. Such as the use of 6% carrot juice, tomato juice 8%, 0.04% ascorbic acid as a nutritional environment and strain the liquid medium for comparison study conducted by Liu Dongmei, who proved to contain 2.5 times the nitrogen source Lactobacillus casei have some positive impact on the growth, results showed that the viable count of Lactobacillus casei 109cfu/m L[7]. Because the host immunization, nutrition, disease prevention and other significant probiotic effect, Lactobacillus casei become a core exploration and production strains.In this study, WIC biologics which in plant waste resources used in growing conditions bacteria, find the optimal culture conditions of Lactobacillus casei bacteria and optimal growth conditions.(1) Using response surface experimental design, analysis of the results obtained at a temperature of 39.51±0.06 ℃, medium initial p H 6.16±0.04, 1.34±0.03% inoculation, WIC biologics at a concentration of static culture environment, 12.56±0.04 mg / m L of next, OD610 nm values of Lactobacillus casei reached 2.227±0.05, the number of colonies of up to 7.3 × 109 cfu / m L.(2) In order to increase the number of live Lactobacillus casei, with the optimum response surface analysis method by shaking culture: the temperature of 38.96±0.04 ℃, medium initial p H 5.98±0.02, inoculation was 1.19±0.04%, natural WIC biologics at a concentration of 12.62±0.03 mg / m L as the basis of the training process can be added 15% Na2CO3 solution, and centrifuged optimum conditions for high-density culture 4000 r / min 20 min and shake bottled liquid amount is 100% of the optimal culture proliferation of cultured bacteria by 4 000 r / min 20 min after the highest yield, not only to strengthen the nutrient medium, also added lye to buffer media within the acid, lactic acid bacteria growth and to provide adequate nutrition is very well prevent excessive lactic acid bacteria "autolysis." Meanwhile centrifugation conditions were explored to ensure a high harvester rates and higher viable cells.(3) The standard strain Lactobacillus casei and WIC biologics sources for comparison, at a p H of 1.5 to 4.5 artificial gastric and intestinal presence of viable cells in culture have 3 h after reaching a high the viable count 1010 cfu / m L, and it can also be grown at 7.5% high salt conditions. Description These two Lactobacillus casei have better adaptability and WIC biologics derived Lactobacillus casei bacteria than the standard body’s digestive tract can adapt to the environment, as probiotic functional food research and development, in the food industry has a certain application.WIC biologics activity of microorganisms in the preparation is small, this experiment will WIC biologics is applied to the growth of Lactobacillus casei, a combination of different culture conditions, response surface experimental design, and to find the optimal conditions for the most gifted enrichment conditions, designed to develop a new culture method of promoting the growth of Lactobacillus casei provide a basis, and Lactobacillus casei on WIC biologics from the adaptability research, not only efficient use of natural plant resources and to protect the global environment has made a contribution, it also provides a theoretical basis for the application of WIC biologics preparation source of Lactobacillus casei. |
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