Research On Key Technologies Of Cell Chip Detector Based On The CCD

With the continuous development of society and the improvement of people’s living standard,people have put forward higher requirements for medical level. But there are still many diseaseswhich can not be effective detected and detection methods which are under-developed in the scienceand technology highly developed era. Leukemia detection is the one with a representative.Thedetection of leukemia mainly depends on the flow cytometer at home and abroad. The detectionequipment not only is expensive but also usually can not complete detection of all types ofleukemia.Research on medical theory based on leukemia immunophenotyping has tended to bemature, thus an efficient test equipment become inevitable and will has important significance to thedetection and treatment of leukemia.According to the detection mechanism of leukemia immunophenotyping, considering the functionalrequirements,thesis puts forward the overall design scheme of cell microarray detection. In addition,according to structure of the equipment, paper proposes and realizes each module in the design indetail, for example putting forward related motion control method by the analysis of motion schemefor every part of the system, realizing the serial communication between upper and lower computer,discussing the control of temperature,achieving chip cleaning and dyeing, realizing the automaticadjustment of light intensity based on PWM and PID algorithm aiming at the problem of imagebrightness of different chip, developing a database system combining the data preservation andmanagement problems by using SQL Server. The key of the system development is the design ofCCD subsystem.Combining the computer vision technology, thesis looks deeper into the realizing ofautomatic focusing system, detection point automatic calibration,cell microarray image automaticmosaic,computer-aided diagnosis and so on in the Visual C++development environment.We have completed the building of the hardware platform and the associated control programs havebeen designed and debugged.In addition,we verify the feasibility and applicability of softwareautomatic focusing, automatic calibration and other related functions by the system which has beenbuilt.