QTLs Analysis Of Height In Soybean Using Residual Heterozygous Lines

Soybean plant height is one of the important factors affecting yield, also is one of the important agronomic in traits conventional breeding of the plant type breeding, so the genetic mapping of plant height has important theoretical guidance and application value of soybean breeding high-yielding varieties.This study was to Jidou 12 x Xinbada 2 hao get BC2F5 generation backcross, hybrid plant selection and the formation of the three remaining heterozygous, respectively RH4, RH6 and RH14, as experimental material. Using of Composite Interval Mapping on soybean plants and other traits of QTL location analysis. To clear the position and effect of Plant Height QTL, relations with other traits, genetic background and environment interaction, and the dynamic development of different stages of Plant Height. The main results are as follows:1. Using Jidou 12, Xinbada 2 hao and three individuals of 231 pairs of primers were screened,11 of the markers were polymorphic in Satt286-Satt307 section, and build 11 markers of the linkage map. In the three RH groups to the control plant height were detected QTL. In RH4 andRH6 populations, Plant height QTL detected the location of the maximum LOD value coincides with the Satt489 marker, genetic contribution rate of 65% and 66%. In the RH14 population, detected the highest LOD value of plant height QTL in marker Satt557, the contribution rate of 42%. Although the target population in RH14 plant height QTL marked the first two groups different, but the difference between Satt489 and Satt557 markers of 2cM. The results show that QTL carrying Jidou 12 played the role of the plant height increased.2. In RH4, RH6 and RH14 populations, detected with Flower time linked to QTL coincide with Plant height. QTL of controlling plant height while controlling Flower time, Maturity date and First pod height. Genetic contribution of Flower time is heigtest in RH4, RH6 and RH14 population, the rate of 71%,81% and 79%. Although detected QTL of Maturity date, the high end of pod,100-grain weight,10-weight and Protein traits, but the low rate of genetic. 3. Result of locating in different environments showed that positioning the QTL in different environments have differences in three RH populations. In addition to the Hainan environment don’t locate to Plant height, Nod number and internode length QTL in RH6 population, the other populations are locate to. Show genetic stability of these trait is good, and can be stable expression。But the same traits in different environments have a certain genetic differences in the contribution rate. Traits of Oil, Protein and 100-grain weight in only one or two traits detected in one environment, and the genetic contribution rate is relatively low.4. Plant height QTL is verificated in five different populations. Four populations (Jidou 12×nf 58, Jidou 12×Ji Huang 13, Jidou 12×Williams, Jidou 12×Flora) were detected Plant height QTL in the Satt286-Satt307 section.5. Results of unconditional QTL position show that Plant height, Nod number, internode length of the QTL were detected in 59 d and 74 d after sowing. Results of the conditional QTL position show that, Plant height, Nod number and internode length associated QTL were detected to in 37 d-59 d after sowing. It is show that Plant height, Nod number, internode length of the QTL begin to express in 37 d-59 d after sowing. This period is the Flower time of soybeans, is the fastest growing period of plant height, and the results of phenotypic analysis of the data exactly.