Preliminary Study On Some Special Nutrients And Potential Harmful Components In Agaricus Bisporus

This research investigated the cultivation modes and methods of handling mushrooms after harvested of the nation’s mainly mushroom producting areas, which are Shanghai, Fujian, Zhejiang, Hubei and Shandong, from September 2009 to March 2011. We collected 30 paris Agaricus bisporus samples. All samples were freeze-dried, ground to powder and detected and quantitatived several special nutriute ingredients: 5’-guanosine monophosphate and 5’-inosine monophosphate, mannitol and trehalose, some harmful ingredients:agaritine, putrescine and cadaverine were also detected, using liquid chromatography. We studied the storage testing of post havrested mushrooms from Shanghai, under 4℃and 20℃. The changes of agaritine, putrescine and cadaverine, 5’-guanosine monophosphate and 5’-inosine monophosphate, mannitol and trehalos of Agaricus bisporus were detected at different days. The aims of this research were at the basis of underground values of the contents of several special components to provided informations for effective prevention, reduction or elimination of all potential quality and security risk factors and clear the management of production quality and security of "critical control points". On other hand, give theoretical basis for maintaining the contents of special nutritent and taste compontents.1. Results of determination of special ingredients of 30 paris Agaricus bisporus samples from different areas after frieeze-dried showed that the agaritine content of mushrooms was 155.6 mg/Kg - 934.4 mg/Kg. Samples of number Shanghai 3,4,5 were not detected putrescine, while the others contain a certain amount of putrescine. And all samples were detected with some cadaverine. The average contents of 5’-guanosine monophosphate and 5’-inosine monophosphate were 0.10 g/Kg and 0.39 g/Kg dry weight. The mainly soluble sugar in Agaricus bisporus is mannitol, followed by trehalose and glucose. The study about the 5’-nucleotides and agatitine content in different strains of Agaricus bisporus showed that 5’-nucleotides and agatitine content are affected by the type of strains.2. Changes of special components in Agaricus bisporus during 4℃and 20℃storage showed, at the condition of 4℃, the content of agaritine was up to 7865.5 mg/Kg at day 6, while down to 5350.3 mg/Kg at day 10; putrescine appeared at day 4 and up to 234.1 mg/Kg at day 10; content of flavor nucleotids was up to 1.26 g/Kg at day 6; changes of mannitol and trehalose were little during storage. While storaged at 20℃, the content of agaritine was down to 2374.0 mg/Kg at day 10; content of putrescine was increased to 1933.2 mg/Kg at day 10 from 0 mg/Kg at day 0; flavor nucleotids was up to 1.19 g/Kg at day 2; after 10 days storage, about 55% of mannitol and 47% of the trehalose was metabolized.