Preliminary Analysis On Combined Effects Of Six Genes To Pig Backfat Thickness Traits

Nearly three decades, China has been committed to the cultivation of new varieties of swine, high-quality lean pork breeding objectives focused on the improvement of backfat traits. With in-depth research on fat genes, many of the features gene is verified, not only can help us understand the growth of pigs, but also for marker-assisted selection of great significance. According to previous studies in this thesis, we screened for genes associated with backfat thickness traits and in-depth study. By BLUP and MAS to explore the effects of multiple genes combined genotypes.The main results were listed as follow:1. Early in the Tongcheng group,we found in the backfat thickness and significantly related to the SNP, in the Yorkshire group for further verification. Association analyses revealed the significant associations(p<0.05) between the MC4R and CTNNBL1 polymorphism and backfat thickness at last rib,average backfat thickness over three positions;The DGAT2 polymorphism was significantly associated(p<0.05) with average backfat thickness over three positions;The CMYA1 polymorphism was significantly associated with backfat thickness at shoulder(p<0.05).The JHDMIA polymorphism was significantly associated with backfat thickness at last rib.(p<0.05).2. Work on the same traits of multiple genes, we performed association analysis of gene interaction.The MC4R-CTNNBL1 and DGAT2-CTNNBL1 interaction was significantly associated backfat thickness over three positions(p<0.05);The MC4R-JHDM1A interaction associated backfat thickness at last rib(P<0.05).3. The pig specific primers were selected from the pig EST contig constructed according to the corresponding human cDNA.The cDNA of SH2B1 were successfully cloned through the PCR amplification from Yorkshire pig genomic DNA by using the primes mentioned above. Detection of SNPs in the amplified fragments of SH2B1 gene was carried out and found a G/A mutation in the sixth intron. The SH2B1 polymorphism was significantly associated with backfat thickness at shoulder.