SSR Single And Double Primer PCA Main Rice Germplasm Resources In JiLin Province Related Analysis On The Application Of Research

This research based on analysis of genetic diversity and genetic relationship by themorphological markers and SSR. The object is42kinds of different rice varieties. The mainresults are these things.1.The morphological markers divide the objects into3groups. The genetic distancewithin groups is little, but the genetic distance within groups is obviously. The genetic distanceis between0and25.There are3parts of rice varieties divided by the genetic distance at15.Group A1：Tong He838、Tong yu403、Dong2、Jiu66、ChangXuan12、Tongjing788、JiNian9、JiNongDa838、KongYu131、YunGuang、3308Riben、JiuDao58、YunLangXiang、JiJing807、SongLiao6、JiNongDa868、Tong35、JiNongDa858、LiaoXing8；Group A2：JiNongDa808、XinDai、BaiDao8、WuYouDao、NongLin315、JiJing108；Group A3：SongJing9、Tong925、TongHe838、JiKeDao512、TongYuan515、JiJing510、ChangBai25、ChangBai21、FuDan3、JiJing88、NongDa31、YiTong2、HongKe8、Lv0659、GuNian2、DongDao2。2．Single primers of SSR markers at genetic distance15divide the materials into3classes:Group A1：YunGuang、WuYouDa、JiNongDa808、YunLangXiang、ChangXuan12、长ChangBai21、JiJing108、JiNongDa838、ChangBai22、LiaoXing8、JiJing88、BaiDao8、NongLin315、JiNian9、XinDai;Group A2：TongYuan515、GuNian2、JiKeDao512、3308RiBen、JiJing510、JiJing807、NongDa31、TongHe838、ChangBai25、Dong2、JiuDao58、TongXi925；Group A3：HongKe8、SongLiao6、JiJing88、TongYu403、FuDan3、JiuDao66、TongJing788、KongYu131、YiTong12、RiBenQing、Lv0659、JiNongDa858、JiNongDa868、JiNongDa30。3．Single primers P1of SSR markers at genetic distance15divide the materials into3classes:Class A1：Jiu66、WuYouDao、YiTong12、YunGuang、Jiu58、Lv0659、SongLiao6、GuNian2、DongDao2、KongYu131、NongDa31、HongKe8、TongYu403；Class A2：TongYuan515、ChangBai25、JiNongDa30、JiNongDa808、TongXi925、JiKeDao512、TongHe838、JiJing510、JiNongDa858、JiJing807、JiNongDa838、JiNongDa868、SongJing9、ChangBai22，BaiDao8；Class A3：NongLin315、FuDan3、XinDai、3308RiBen、RiBenQing、ChangXuan12、 JiJing88、YunLangXiang、LiaoXing8、ChangBai21、JiJing108、Tong35、TongJing788、JiNian9。4．Single primers P2of SSR markers at genetic distance15divide the materials into3classes:Class A1: DongDao2、Jiu58、JiNongDa30、TongXi925、KonguY131、WuYouDao4、LiaoXing8、JiJing807；Class A2: TongHe838、Lv0659、TongYu403、NongDa31、Tong35、JiNongDa868、YunGuang、 BaiDao8、3308RiBen、 SongLiao6、 JiNongDa808、 TongYuan515、FuDan3JiNongDa838；Class A3: ChangBai21、JiNian9、TongJing788、SongJing9、JiJing108、ChangXuan12、GuNian2、ChangBai22、Jiu66、XinDai、JiJing510、YunLangXiang、RiBenQing、ChangBai25、NongLin315、JiKeDa512、JiJing88、YiTong12、HongKe8、JiNongDa858.5.The results of PCR and agronomic traits analysis are these. Pairs of primers clusteringdivided42material into three groups which has higher inosculation with single primers P1are46.2%,70%and61.7%respectively; With single primer P2to three groups of alignment are29.3%,41.6%and29.3%respectively, and phenotypic clustering by the three major groups ofalignment were30.7%,51.2%and55.2%, respectively.Primers P1clustering divided42material into three groups which has inosculation withsingle primers P2are47.5%,37.6%and53.3%respectively; With the phenotypic clustering bythe three major groups of alignment were46.2%,22.5%and56.3%respectively.The phenotypic clustering divided42material into three groups which has inosculationwith single primers P2are47.5%,37.6%and53.3%respectively.Through pairs of primers PCR amplification and single primer PCR amplification at thesame condition can remove false positives phenomenon caused by single primer amplificationand get more information on the genetic diversity in the study of SSR molecular markers. Thisresearch has important application value on genetic diversity and evolutionary relationships,germplasm identification and marker-assisted selection breeding.6.The suitable reaction system is20μL.It contains2μL of10×reaction buffer2,1μLofDNA,1μLof primers,0.5μL of dNTPs,0.25μL of rTaq and15.25μL of pure water.7. Morphological markers has differences and obvious genetic diversity between differentvarieties. At the same time, more quantitative characters, more richer genetic diversities.However, due to the greatly influenced by the external environment, morphological markers ingenetic relationship between each type of conclusion is not accurate, the genetic distancebetween varieties are difficult to match the physical distance.