Genetic Diversity Analysis Of Radish (Raphanus Sativus L.) Germplasm With Molecular Markers

Radish (Raphanus sativus L.) is one of the important vegetables cultivated worldwide. China is one of area of radish origin and the germplasm resource very abundant, intensive study and rational utilization Is particularly important for breeding work of radish germplasm resource.On the preduction at the present, requirement of new fertility, good fitness, (and commodity, quality Requirement of new cultivar fertility, Widely suitable, multi-resistant and Good commercial,good quality, but the realization of the target depends on the mastery of the radish germplasm resources quantity and quality and the reasonable use. The study on the genetic diversity of radish can benefit the conservation,classification,identification,genetic enhancement and effective utilization of the radish germplasm resource. Molecular marker which can detect the genetic diversity at DNA level,is a powerful tool to study genetic diversity. Using molecular marker technology research and breeding materials of genetic diversity will provide radish classification, origin, genetic relationship with the basis of molecular level, And can be used as a selection, the progeny of radish genetic breeding to determine a rapid analysis method, At the same time for the construction of core radish germplasm repository to provide reliable technical support. This study adopted the ISSR markers, RAPD markers and the RAMP markers, Combining with the system cluster analysis, Of 32 radish germplasm genetic diversity and genetic relationship were studied.1. From 35 primers screened eight good polymorphic random primers, the ISSR analysis of 32 radish germplasm, amplification produced 82 bands, including polymorphism with 69, polymorphism percentage is 84.1%, each primer an average of 8.6 bands polymorphic primers. System clustering analysis 32 germplasm can be divided into four groups, the first big groups consists of 28 species, is divided into three subgroups, second largest groups of MTHS alone, third largest groups composed of LYDHP and LSHLB two materials, red one red hearts of YZLB alone as the fourth largest groups.2. From 48 primers screened 6 good polymorphic random primers, the RAPD analysis of 32 radish germplasm, a total of 58 stripe amplification, polymorphism of 50, polymorphism percentage is 86.2%, each primer an average of 8.3 bands polymorphic primers; System clustering analysis were used to material group is divided into four categories, the first big group including 16 material, and can be divided into four subgroups, the second largest group of 11 copies of materials, and can be divided into three subgroups, the third largest group includes four material, give priority to with green radish, The fourth largest group consists of MTHS separate from Japan.3. From 96 pairs of primers screened eight pairs of primers, of 32 radish germplasm are analyzed in the RAMP, amplification produced 122 bands,113 polymorphism bands, polymorphism percentage is 92.6%, average each pair of primers produced 14.1 polymorphic bands; System clustering analysis will be divided into three groups, the germplasms in the first group includes 29 of material, the first group consists of QTQ and FLXX 2 material composition, the third group consists of MTHS material separately.4. Comprehensive results of three kinds of marker cluster analysis showed that a single tag and comprehensive clustering sequence is not completely consistent, but they are almost the same classification and morphological markers.MTHS material can be separated clustering; CBL, FT type radish coming from the surrounding area chongqing can be basicly clustered same groups, their genetic relationship is very close; JG and BJSG, JSNBZ and ZDC and YCLBF, LSHLB and LYDHP are all be clustered together at a certain similarity coefficient, the shape of the root is showed very close relationship with clustering. Three comprehensive clustering tags, more intuitive to reflect the relationship among the germplasms, its consistency with horticulture traits is higher than any single marker. And in this study integrated clustering analysis results to the RAMP mark the closest.