The Research Of Wnt5a And FGF4Gene’s MRNA Expression In The Goose Feather Follicles And Feathers Development

In this experiment, Jilin White Geese were chosen as the experimental material, using theskin and feather from the dorsum of Geese which are12age of embryonic period(E12) and127days of post-natal stage(D127), applying Fluorescence quantitative PCR(FQ-PCR) andimmunohistochemistry methods to analysis change law of Wnt5a and FGF4gene expressionand location of follicles in different periods quantitatively and qualitatively. In order toinvestigate effects of these two genes on goose feather follicles in embryonic period andpost-natal stage.The overall trend of Wnt5a and FGF4genes:E12-E18, high gene expression of Wnt5a inhibited β-catenin gene expression, E18-E30,Wnt5a gene expression level decreased, primary follicles and secondary follicle developedactively, the depth increased significantly, E30-D7, Wnt5a gene expression level decreasedslightly, as the development of primary follicles is almost completed, since there was almost nochange in the depth, but secondary follicles are developing. D7-D23, the expression wasgradually increased to highest-level, high expression of Wnt5a inhibited the growth of hair shaft,hair shaft grown lowly, D23-D37, Wnt5a gene expression level decreased slightly, D37-D49,Wnt5a gene expression level increased slightly, D49-D58, gene expression of Wnt5a decreased.D57-D76, gene expression increased. D76-D84, highest level, after D90, Wnt5a gene expressionis almost completed.Gene expression levels of FGF4increased gradually in E12-E16, inE16-E18, the expression reduced, E18-E22, gene expression increased, E22-E24, genedecreased, E24-E30, gene expression is almost completed and in D7-D70, FGF4promoted thefeather increasing developed actively, gene expression increased D70-D84, gene decreasedD90-D127, gene expression increased.Mapping expression results of Wnt5a and FGF4genes:E18, the expression of Wnt5a gene were positive and massive in inner root sheath andouter root sheath of barb ridge and hair follicle base(dermal papilla and medulla). E29, Wnt5aalmost did not express in primary follicles, only a small amount of expression are in the medulla,D23, there were a little expression of FGF4at periphera plate of primary follicles. D57, positiveexpression were in barbs ridge. D69, Wnt5a gene were positive and massive in hair follicle. D90,only a small amount of positive expression were in barbs ridge. E18, FGF4only expressed atperiphera plate of secondary follicles, while expressed strongly in the primary follicle; E23, FGF4were positive in periphera plate of follicles, a little expression of FGF4at barb ridge. D13,the expression of FGF4was in periphera plate of follicles, there is a little expression in barbridge; D35, there were a little expression of FGF4at periphera plate of primary follicles, andthere were massive and positive expression in periphera plate of secondary follicles; D69, therewere positive expression at hair follicle. D90, FGF4expressed in the periphera plate of follicles,barbs ridge. It revealed that these two genes play a functional role in starting and developingprocess of follicles.