Cryptocaryon Irritans Infection LAMP Detection And Analysis Of Pseudosciaena Crocea

In recent years, with the rapid development of Pseudosciaena crocea breedingindustry, the frequency and scale of Pseudosciaena crocea cage aquaculture diseasesoccur every year uptrend, where Pseudosciaena crocea “white spot disease” hasbecome a hazard one of the most serious epidemics.“White spot disease” tostimulate Cryptocaryon irritans. After a large number of sick fish infected with theparasite, there will surface, fin and gill flap covered with visible white dot the size ofparasites and cysts, activities abnormal epithelial hyperplasia, difficulty breathing,mechanical damage and other illnesses. In this paper, sea cultured Pseudosciaenacrocea,fish tissue infections stimulate the insects Cryptocaryon irritans trackdetection, elaborated during the period2012to2013, to stimulate the Cryptocaryonirritans infection of cultured Pseudosciaena crocea law; studing the effects oftemperature and salinity of sea water physical and chemical stimulation ofPseudosciaena crocea factor infection; Analysing the differences betweenCryptocaryon irritans infection in different fish tissues; Using molecular biologytechniques, to build and improve the LAMP detection, for Pseudosciaena croceastimulate Cryptocaryon irritans disease prevention and control technology researchand practice the stimulation of covert nuclear insect pathogen detection, monitoringprovides the theoretical basis and experimental evidence.This article rely on automatic circulation chain substitution reaction, byidentifying the target sequence on four specific primers of area and has a chainsubstitution of DNA polymerase in magnesium2+concentration of2mmol-1, thereaction temperature is61℃~66℃, and outside the primer concentration2:1~6:1,60min under the optimized conditions of reaction time, amplification is specific LAMPtrapezoid strip product, build perfect LAMP test. This detection method is simple,highly sensitive, high accuracy, practicality, not only the incidence has not beendetected in both infected diseased fish, but also to detect the onset of fish, providinga new method for the rapid detection of Cryptocaryon irritans.Research breeding sea water to Cryptocaryon irritans. infections stimulate implicitimpact on the Pseudosciaena crocea: summer (late May-early July) and the summer (lateAugust-early October) is to stimulate Cryptocaryon irritans infections peak of Pseudosciaena crocea, water becomes one of the effects of stimulation occurs most Cryptocaryon irritans sisfactor, changes in water temperature directly affects the large yellow croaker infection,stimulated an implicit warning of Cryptocaryon irritans sis prevention through temperaturetemperature monitoring.Research breeding sea salinity to Cryptocaryon irritans. infections stimulate implicitimpact on the Pseudosciaena crocea:2012~2013breeding sea salinity fluctuation range:between20‰~30‰, this interval within tolerable limits stimulus Cryptocaryon irritansrange is more appropriate stimulate reproduction, growth, development, life.Research to stimulate hidden nuclear insect body infection in different tissue to the fish:when the irritation of the Pseudosciaena crocea Cryptocaryon irritans infection, gill tissueinfection first, and quickly a large number of parasites, in a short period of time, gill tissuewill be severely damaged, and breathing difficulties, eventually die. And gill tissue infectionrate is much higher than other organizations such as skin, muscle, so when fishing at ordinarytimes ahead to stimulate hidden nuclear insect disease prevention and control.Pseudosciaena crocea in the lower cage breeding sea water inside and outside thenucleus to stimulate the Cryptocaryon irritans detection probability, and can cage thedetection period, no significant difference in the outside, and the detection rate in fish rhubarbno correlation. Not for the Pseudosciaena crocea breeding sea worm infections stimulateCryptocaryon irritans implicit indication as judge.