Study Of Mutant Genetic Identification、Gynogenesis、Estradiol Induce And The Effect Of Follistatin2to Megalobrama Amblycephala

Megalobrama amblycephala known as Wuchang fish, bream, originated in Chinasome lakes in the middle reaches of the Yangtze River, is one of the importantherbivorous fishes endemic to china. After years of breeding, now has a blunt snoutbream thoroughbred breeding, however, endless; to better meet the growing marketdemand, critical breeding and research of Megalobrama amblycephala thoroughbred,the experimental effect of gynogenesis and estradiol in this study were based on theidentification of mutants of blunt snout bream Megalobrama amblycephala, induction,and provide certain data the basis for the breeding of Megalobrama amblycephala.In the study, five red bream mutant Megalobrama (two males and three females,)discovered in the natural environment were identified. Seven countable characters,eleven measurable characters and twenty framework were measured, based on witchPrincipal component analysis has worked out with the data of the six genus Parabramisand Megalobrama measured by laboratory, the results showed that, the five red breammutants clustering separately in measurable characters, and near Megalobramaamblycephala; the study found that microsatellite markers were different between thefive red bream mutants and Parabramis pekinensis, Megalobrama terminalis,Megalobrama terminalis, Megalobrama pellegrini, Megalobrama hoffmanni, butsimilar with Megalobrama amblycephala; finally, clustering analysis by the sequencesof COX1gene and COX2gene of the five red bream mutants and other closely relatedspecies, founded that the relationship of the five mutants close to Megalobramaamblycephala, the five mutants belongs to the spontaneous mutant of Megalobramaamblycephala, the red Megalobrama amblycephala can provide valuable materials forthe study of genetics and breeding of Megalobrama amblycephala.Through the investigation of the same size specifications of Megalobrama amblycephala seedling emergence of15days respectively with the concentration of0,10,30,100,200,300μg/L estradiol soaking treatment for60days, and then cultured innet cage, the survival rates were73.3%,87.5%,40%,41.6%,52.5%,4.2%; in cageculture after1months, to measure the concentration of estradiol treatment group ofbody length, body height and weight as the first measurement data, measure body length,body height and weight again after second months cage culture as the second measureddata. By the2sets of data, calculate the average growth of body length, body height andweight values were found in all the groups, numerical estradiol treated was higher thanthat of the control group. Among them, the estradiol treatment conditions of100μg/Lconcentration, body height growth quick than the control group, the difference was notsignificant; at a dose of10μg/L treatment conditions, body height growth quick than thecontrol group, there were significant differences; while in the experimental group, thedifference very significant. Experiment demonstrated that estradiol treatment at theexperimental concentration has significant inhibitory effects on the growth of seedlingsof Megalobrama amblycephala.Experimental research on the transformation of FST2gene to Megalobramaamblycephala, found that the FST2gene transferred into positive individuals is very few,survival rate is very low, the survival of positive individuals and control measurementgroup of body length, body height and body width, found that a high/body length andbody width/length ratio were higher than the control group, the FST2gene had obviouspromoting effect on muscle growth.