The Polymorphism Analysis With AFLP In Cytoplasmic Male Sterile Liner And Maintain Liner In Buds Of Owen-sugar Beet

So far, the sugar beet has become one of the most important sugar crops whichaccounts for40%of global sugar production. For enhancing per unit area beet yield,cytoplasmic nuclear male sterility usually used as obtaining hybrids based on heterosis.Currently, among F1hybrids used for cultivate beet, vast majority of these materials areOwen cytoplasmic male sterile, this will result in cytoplasm type is over single and leadto disease, so beet breeders are faced with a challenge that they must look for new typesof cytoplasmic male sterility. Whereas, due to the limit of technology, the breedingprocess of male sterile liner and maintain liner can not achieve satisfied result. So wehope will find key genes by molecular biology technology, using these polymorphicfragments can rapidly identify which sugar beet is cytoplasmic male sterile liner andwhich is maintain liner, shorten breeding period and finally applying to practicalproduction.In this paper, leaves and buds of Owen cytoplasmic male sterile liner and maintainliner (DY5-CMS and DY5-O) were used as materials, respectively, leaves were usedAFLP and later were used cDNA-AFLP molecular marker, and the level of AFLP isgenome, while cDNA-AFLP is transcription. In this experiment, finding16differentfragments in leaves. And only7sequences were successful recovered, but no fragmentcan obtain gene annotation. While cDNA-AFLP approach was used to research the levelof transcription polymorphism analysis in buds, recycling35bands in the maintain liner,25bands inn cytoplasmic male sterile liner. And18specific different expressedfragments were successful comparative analysis though NCBI-Blast, and retrieved thecorresponding homologous genes which were known function, respectively: encodingprotein kinases, encoding hypothetical protein, encoded transporter G family member,encoding vacuolar-sorting receptor, encoding cytochrome c1, encodinggalacturonosyltransferase, encoding transcription factor, encoding DNA-directed RNApolymerase, encoding the ubiquitin-conjugating enzyme genes. The remaining12bandswere successfully posited on nuclear genome chromosome of sugar beet.So, we can make a conclusion: the difference of Owen male sterility sugar beet ongenome is little, while difference on transcription is more effective and can make a lotof significant different fragments, and these fragments not only can explore themolecular mechanisms of male sterility in sugar beet but also apply to practicalproduction after deeply research.