Proteomic Analysis Of Florescence Differentiation Between Cytoplasmic Male Sterile Line And Maintainer In Sugar Beet

Sugar beet (Beta vulgaris) is an important crop plant that accounts for30%of theworld’s sugar production annually. In order to make best advantage of sugar beetcytoplasmic male sterile lines in heterosis utility and improve the sugar yield of Sugarbeet, it is very important to explore the mechanism of cytoplasmic male sterility(CMS). We have used the proteomic approach to identify differentially expressedproteins in the Sugar beet male-sterile line(DY5-CMS)and its maintainer line(DY5-O)buds and the objectively analyzed their roles in normal pollen development whichwere divided into three stages(stamen primordium differentiation stage, metaphase anduninucleate stage) and in male sterility by using2-DE and MALDI-TOF-MS. Severaldistinct proteins were detected at the different stages of pollen development. Theseresults may contribute to further understanding of the mechanisms of CMS in sugarbeet.(1) Six distinct proteins were identified at the stamen primordium differentiationstage in the DY5-CMS. Five of them are related to the plant energy and respiratorymetabolism and these five proteins were all up-regulated in the sugar beet male-sterileline. It inferred that ATP synthase were hydrolysed lead to energy is shortly supplied.Therefore, these proteins were over expressed in the male-sterile line, but it still couldnot meet the demand for energy in the process of plant metabolism.(2) On the metaphase, three protein spots which related to sterility was detected,they are all very important enzyme in the TCA, cell cycle regulation and catalaseremoval process. These proteins all appeared in male-sterile line, but absent in that ofmaintainer line. It inferred that the over express of these protein caused thesemetabolic disorders in the process lead to male sterility.(3) Severn distinct spot were identified at the uninucleate stage, five of them wererelated to the plant photosynthesis. This conclusion on the one hand revealed that thephotosynthetic function of anther began to development since mononuclear stage. Onthe other hand, it revealed that the plant male-sterile are related to the photosynthesis.(4) In order to make the experiment more rigorous, the flowering proteins of leafwere compared between the sugar beet male-sterile line DY5-CMS and its maintainerDY5-O. Severn distinct spot were identified, these protein are more related to thevegetative growth of plants or resistance, their relationship with the sugar beetcytoplasmic male sterility (CMS) remains to be further research.In conclusion, the distinct proteins are mainly related to the metabolism of energyand respiratory at the prophase of pollen development(contain stamen primordium differentiation stage and metaphase). But in the uninucleate period of pollendevelopment and flowering leaves, the distinct proteins are mainly related to thephotosynthesis of plant.