Comparison Of Germinating Protocorm And Endophytic Fungi Of Dendrobium Sinense Sowed In Local And Laboratory Condition

Many kinds of Orchidaceae Plants have the extremely high ornamental value and medicinal value. However, Wild Orchidaceae Plants resources are increasingly scarce for many reasons. Dendrobium sinenses which has the low natural propagation coefficient and slow-growing, is native only to Hainan. Orchidaceae Plants are the typical mycorrhizal plants, symbiotic fungi are important for growth and development of Orchidaceae Plants. And the survival and growth of fungi have a close relation with its environment. It is an important significance that studies the symbiotic germinating fungi for the local protection of Orchidaceae, the propagation of Laboratory condition and etc. Therefore, in this research, the number, time and other data of germinating seeds are investigated on Dendrobium sinenses which sowed on different epiphytic host barks in original habitat and laboratory condition. And comparing requirements attributes of moisture for endophytic fungi by separating protocorms which germinated in original habitat and laboratory condition. In addition, the difference of germinated protocorm and endophytic fungi which sowed in original habitat and laboratory condition is also analysed. The results of this paper indicate as follow:(1) When the seed sowed in the original habitat and laboratory condition, protocorm is easiest germinated on Rhododendron moulmainense. Two and a half months after sowing, the germination can be observed. Four months later, the number of protocorm peaked. At the laboratory condition, parts of protocorm begin to die out. At the original habitat, the number of protocorm begins to stabilize after six and a half months. There is no germination on Cyclobalanopsis blake at the original habitat. And protocorm can germinated on Cyclobalanopsis blake at the allopatric laboratory condition. For Syzygium buxifolium and Rhododendron moulmainense, they have germinated protocorm in both original and laboratory condition. Conversely, there is no germination on Exbucklandia-tonkinensi and Pinus massoniana.(2) There are10types of endophytic fungi which found from the germinated protocorm of dendrobium sinense, including4types in original habitat, and6types in laboratory condition. And no two types of these endophytic fungi are similar. It is means that there is a difference of symbiotic germinated protocorm at local and laboratory condition.(3) For the endophytic fungi of protocorm. When the endophytic fungi has a slower-growing in normal cell cultures, the growth rates are positively correlated with moisture. Rather, when the endophytic fungi has a faster-growing, moisture has little effect on the growth rates. In addition, the moisture of culture has a significant impact on compactness of hyphaes. The average growth rate of endophytic fungi of germinated protocorm in original habitat is greaterthan in laboratory condition. And the difference decreased with the increasing of moisture.