Effects Of Midazolam On Fetal Rat Nerve Cell In Vitro

The purpose of the experiment:Midazolam is a classic short-acting benzodiazepine class of drugs, which is typically used in clinical preoperative sedation. To clear the influence of midazolam on neurotransmitter amino acids, monoamine neurotransmitters, ATP enzyme transmembrane signal transduction systems, cAMP signal transduction pathway and NO-cGMP signal transduction pathway in the central nervous system, and to explore the major target point during the anesthetic and the possible paths to participate in anesthesia.The method of experimental:In this experiment, the90fetuses, as experimental subjects, were divided into three big groups and each big group were devided into3small groups again, giving big groups different doses, Low-dose group3.41×3μg/ml, middle dose group5μg/ml. high-dose group60μg/ml by direct cell mode of administration, and sampling at051015202530456090120min.We use mass spectrometry to measure monoamine neurotransmitters, ELISA to test the content of amino acid neurotransmitters. cGMP and cAMP, moreover, microplate method to measure the activity of ATP enzyme and the content of NO and NOS.The results of experimental:The experimental results showed that:1different concentrations of midazolam make excitatory neurotransmitter up first and down later, oppositely it makes inhibitory neurotransmitter decrease first and increase later:2different concentrations of midazolam make catecholamine neurotransmitter up first and down later, serotonin amine neurotransmitter down first then up3different concentrations of midazolam make ATP enzyme, NO and NOS significantly reduce, make cAMP and cGMP decrease first and then increase.Conclusions:The mechanism of midazolam may be caused by the release of inhibitory neurotransmitters and serotonin amine neurotransmitter, by the inhibition of ATP enzyme, cAMP and cGMP signal pathway system. This study reveals the effects of different concentrations of midazolam on rat neuronal amino acid neurotransmitters, monoamine neurotransmitters and parts of signal transduction pathway, explores the mechanism of midazolam, and furthermore provides a theoretical basis and practical foundation for deeper research on the cellular level and clinical application of midazolam.