The Effect Of Xylazine On Neurotransmitters And Signal Transduction Pathways Of Rat Cerebral Neuron In Vitro

Neurotransmitters and signal transduction pathways of the central nervous system were the important ways to study the mechanism of anesthesia. According to the literature, anesthetic, in vivo experiments, can cause varying effects on neurotransmitter levels and indicators for signal transduction pathway, thus play an anesthesia effect.But there was no related report about the anesthetic effects on neuronal cells in vitro.In this experiment, XYLAZINE was administrated to the rat brain neurons cells in vitro culture system to study its stimulation. By detecting the secretion of neurotransmitters and related indicators of signal transduction pathways, we can study the mechanisms of XYLAZINE anesthesia on the central nervous system and provid a theoretical basis for the clinical application. Moreover, this experiment established the appropriate programs for culturing neuronal cells in vitro, and there was a modest level of number of neurons cell,which had a perfect and high density in the wells. This technique can be used to experimental model of anesthesia mechanism research for a variety of applications. LC-MS/MS method detecting the neurotransmitter was reliable, good accuracy, sensitivity and reproducibility which can be applied in studying the mechanism of XYLAZINE.Experimental results showed that, XYLAZINE can increase the amino acids neurotransmitter Gly significantly which can inhibit neuronal cell secretion in vitro culture, levels of GABA decreased at first and then increased significantly, both of them showed increasing trends; it can also cause significantly lower levels of excitatory amino acid neurotransmitters Glu and Asp secreting by neuron, then gradually recovered to the blank controller; XYLAZINE had obvious inhibitory effect on cultured neural cells secreting catecholamine neurotransmitter(DA and NE) in vitro, which obviously increased the serotonin neurotransmitter content(5-HT and5-HIAA); In the experimental results of signal transduction pathways, the activity of Na+-K+-ATPase and Ca2+-Mg2+-ATPase had been inhibited obviously by XYLAZINE; in the cAMP signal transduction pathway, the content of the cAMP obviously increased initially and then rehabilitated to the levels of the blank controller; in the experimental results of NO-cGMP signal pathway, the content of NO and CAMP and the activity of NOS had been significantly decreased.In summary, we can conclude as follows:the mechanism of XYLAZINE inhibiting central nervous system may be achieved by the promotion on the secretion of GABA,Gly and5-HT in neurons; the sedation mechanism of XYLAZINE may be achieved by the inhibition on the secretion of NE and Glu in neurons; Na+-K+-ATPase and Ca2+-Mg2+-ATPase may be one of the targets of XYLAZINE; The cAMP signal transduction pathway joined in the XYLAZINE of anesthesia mechanism; the inhibition of XYLAZINE to the NO-cGMP signal transduction pathway may be caused by inhibiting the activity of NOS.