Effect Of Phytase Transgenic Maize On Physiological And Biochemical Responses And Intestinal Microorganism Of Bombyx And Ostrinia Furnacalis

The problem of transgenetic food safty is the barrier for transgenosis food spreading. Transgenetic food needs a good deal of security detections. With the long-time cultivating of transgenosis plants, the protein delivered by foreign fene could be accumulated and collected through entering into the food cycle of its grain. In the year of2010, Chinese Academy of Agricultural Sciences had developed Phytase Transgenic Maize with independent intellectual property rights which had large numbers of advantages such as increasing the nutrition using ratio of forage, decreasing the pollution of phosphorus, reducing the cost of production and so on.At present, there is no report about effect of phytase transgenic maize on the related insects. Feeding bombyx with the pollen of phytase transgenic maize and its non-transgenic parent. Feeding asiatic corn borer with the niblet of transgenic maize10TPY005with phytase gene, its parents and non-GM maize. The effect of phytase transgenic maize on the organism of these two kinds of insects were studied by measuring index from three aspects of growing development, biochemical reactions and the diversity of its intestinal micro flora respectively. It enriched the theory research of the ecological security of transgenic plant,meanwhile, it provided reasonable planting judgement for phytase transgenic maize. It also has important theoretical meaning and practical significance.The results were as follows:(1) Feeding bombyx for three ages with the pollen of transgenic maize10TPY005with phytase gene and its non-transgenic parent. The effect of phytase transgenic maize on the organism of bomdyx were studied by measuring index from three aspects of growing development(including survival rates, body weights and food utilization) and biochemical reactions(including protein content, three kinds of detoxifying enzyme activity, and two kinds of protective enzyme activity) and the diversity of its intestinal microfiora respectively. The results showed that there was no significant difference between the bombyx which fed by phytase transgenic maize pollen, its non-transgenic parent pollen and blank control group. Thereby, there was no obvious harm of phytase transgenic maize to the normal physiological activity and biochemical reaction of bombyx.(2) Feeding asiatic corn borer for three generations with the niblet of transgenic maize10TPY005with phytase gene, its parents and non-GM maize. The effect of phytase transgenic maize on the organism of asiatic corn borer were studied by measuring index from three aspects of growing development(including survival rates and body weights), biochemical reactions(including protein content, three kinds of detoxifying enzyme activity, and two kinds of protective enzyme activity) and the diversity of its intestinal microflora respectively. It turned out that there was no significant difference among the asiatic corn borer which fed by phytase transgenic maize, its parents and non-GM maize. Thereby, there was no obvious harm of phytase transgenic maize to the normal physiological activity, biochemical reaction and the diversity of its intestinal microflora of asiatic corn borer.(3) In field experiment, adopting diagonal sampling at five and cutting stalk to investage its target pests from transgenic maize10TPY005with phytase gene, its parents and non-GM maize.The index including wormhole number and worm number of each plant, growing development, biochemical reactions and the diversity of intestinal microflora from the collecting asiatic corn borer. The experimental results showed that transgenic maize10TPY005with phytase gene had no significant effect on the above indicators of asiatic corn borer.