The Function Of PBPs And OrCo In Lymantria Dispar

Gypsy moth, Lymantria dispar (Linnaeus), is one of the most dangerous forest pests in global forest ecological system, which plays an important role in the plant quarantine. When the amount of L.dispar(Linnaeus)grows rapidly, the plant leaves are eat up quickly, which seriously affect the growth of trees and even lead to death. Olfaction plays an important role in the process of chemical information transmission between the insect and the environment. And it also has a certain of development potential in developing lure substances. Therefore, the further study of adult olfactory system is a big breakthrough to control the gypsy moth. Researching the the characteristics, variability, and specificity of odor receptors is important in clarifying the molecular mechanism of olfactory recognition.Firstly, the full-length cDNA sequence of orco in Lymantria dispar (Linnaeus) was cloned using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) and its sequence analysis was also made. Secondly, we have used RNAi technology to successfully silence the olfactory receptor gene of orco and sex pheromone binding proteins gene of pbpl and pbp2. Now the results are summarized as follows:(1) The successful cloning and sequence analysis of olfactory receptor gene orco in L.dispar provides a basis for exploring the functions of the olfactory receptor, the evolution of the orco gene and the mechanism of insect olfaction. It also provides new ways and methods to the biological control of Lymantria dispar (Linnaeus)(2) This experiment was successful to silence olfactory receptor gene of orco and sex pheromone binding proteins genes of pbpl and pbp2with RNAi technology. And the result were tested and verified by electrophysiological experiment and real-time PCR experiment. The lack of these three genes does influence their recognition to sex pheromone. The study preliminary verified the orco gene has similar function with other similar genes of predecessors studying other insects. And found that the three genes have mutual promotion effect, showing that the process of conduction of odor molecules coming into olfactory sensillum in Lymantria dispar (Linnaeus) are similar to predecessors’ studying other insects. But their specific transmission mechanism still needs further research. In a word, the study provides important basis for prevention and control to the gypsy moth.