Study On Tissue Culture And Morphological Of Arisema Amurense Mamim

In this experiment, the Arisaema amurense Maxim.leaves, stems, seeds and other materials to study the morphological characteristics of different gender Arisaema amurense Maxim., the growth pattern of the Arisaema amurense Maxim, stems, leaves and microstructure flowers, seeds, and seed research organization Arisaema amurense Maxim.cultivation and seed germination. The result showed that:1、The annual Arisaema amurense Maxim, no gender differentiation, only vegetative growth,5months early germination unearthed, mid June to stop growing, in early7began to wither, growth period is short, perennial male and female Arisaema amurense Maxim.e Maxim long, growth period was more than one year,5months early germination unearthed, leaves and spathe together from the sheath extraction, male Arisaema amurense6months late stop growing, perennial female Arisaema amurense maxim at the end of6to stop the growth of.2、The annual Arisaema amurense Maxim plant height, leaf size and corm diameters were less than perennial males and females of Arisaema amurense Maxim, and the gap between the larger; perennial Arisaema amurense Maxim male and female plants had little difference, female plants were slightly larger than the male plants.3、The annual Arisaema amurense Maxim stems in epidermal thickness, xylem, phloem thickness thickness and the number of vascular bundles are expressed as minimum, between male and female Arisaema amurense Maxim perennial difference, in addition to the epidermal thickness on the female is slightly lower than the male, the other is slightly higher than that of male female plant.4、Leaf thickness, epidermis thickness of annual Arisaema amurense Maxim leaves, vascular bundle number and main vein vascular diameter as the minimum between male and female, perennial Arisaema amurense Maxim difference, leaf thickness and leaf epidermis thickness of female was slightly larger, the number of vascular bundles and the main vein vascular bundle diameter slightly larger for male plant.5、Arisaema amurense Maxim is a dioecious, male flowers smaller, anthers with2or4pollen sac, as a female flower ovary, the ovule many, is orthotropous ovule.6、B5basic medium than in MS medium in favor of Arisaema amurense Maxim seeds sprouting; auxin IAA than NAA in favor of Arisaema amurense Maxim seed germination; low concentration of auxin and cytokinin combinations (2mg/L6-BA+0.5mg/L IAA) higher concentrations of auxin and cytokinin combinations (5 mg/L6-BA+1.0mg/L IAA) in favor of Arisaema amurense Maxim seed sprouting7、With the gibberellin treatment, Arisaema amurense Maxim seeds in the medium budding required time shortened, the budding rate can be improved.8、Of Arisaema amurense seedlings tubers and leaves were induced, better inducing effect of tuber, induced many buds, in the design of the3kinds of induction medium, the inducing medium No.1medium the most suitable (MS+2.0mg/L6-BA+1.0mg/L2,4-D).9、The Arisaema amurense Maxim seed optimal germination temperature is20℃; plant growth regulator GA3,6-BA and NAA have improved germination potential and germination rate of Arisaema amurense seeds, the GA3concentration was100mg/L, germination potential and germination rate was the highest Arisaema amurense Maxim seeds; GA3optimum soaking time is3h.