Effects Of Interaction Between Vitamin E And Magnesium On Growth And Physiology Ofjapanese Seabass, Lateolabrax Japonicus

A series of studies were conducted to investigated the effects of vitamin E (Vitamin EAcetate)and magnesium (magnesium sulfate) atdifferent levels in diets on the growthperformance, antioxidant capacity, immunocompetenceand contents ofserum ions in Japaneseseabass (Lateolabrax japonicus). Explore the interaction effects of vitamin E and magnesium ongrowth and physiology of Japanese seabass. The results are presented as follows:Japanese seabass (initial body weight were6.1±0.2g)was fed with diets containing threelevels of VE (0,60and200mg/kg) supplementation,each supplemented with three levels of Mg(0,520and1570mg/kg), respectively, for8weeks.The results showed that dietary Mg and VElevels significantly affected the WGR, SGR and FE（P<0.01）of experimental fish, and the peakvalue was observed in60mg/kg VE+520mg/kg Mggroup. Dietary Mg and VE levelssignificantly affected the contents of bodyand muscle lipid of Japanese seabass, and with theincrease of dietary VE, muscle lipidincreased significantly and thendecreased in200mg/kg VEsupplementation group(P<0.05). There were no significant differences in CHO and LDL-Camong Mg dietarytreatments (P>0.05), but with the increase of dietary VE, CHO andLDL-Cincreased significantly and thendecreased. At the same VE levels, SOD in liver increasedsignificantly with the increase of dietary Mg to520mg/kg (P<0.05), and then decreased orleveled off.The SOD in liver of fish fed diets supplemented0and200mg/kg VE weresignificantly lower than those fed60mg/kg VE supplemented diets in0or520mg/kg Mgsupplemented groups (P<0.05). However, with the increase of dietary VE, SOD in liverdecreased significantly in60mg/kg VE supplemented groups (P<0.05).There were significantdifferences in MDA and SOD among all dietary treatments (P<0.05). Furthermore, there werehigher level of SOD and lower level of MDA in liver of fish fed the diet supplemented with60mg/kg VE and520mg/kg Mg (P<0.05). The contents of Mg in the body and muscle wereaffected significantlyby dietary magnesium (P<0.05). The contents of Mn in the body wasaffected significantlyby dietary vitamin E (P<0.05).There were no significant differences in theNa, K, Ca, Se an Zn content in fish body or serum. The results indicated that dietary appropriateMg and VE levels can improve growth and antioxidant capacity of Japanese seabass infreshwater.And there is interaction between vitamin E and magnesium. In conclusion, dietary vitamin Econtent of60mg/kg and magnesium content of520mg/kg are appropriate for Japanese seabass.