Study On The Changes Of Serum Oxidative Stress And Trace Element Content In Peripartum Cows

40prenatal45d cows have been chosen to study the serous oxidative stress and trace elements in perinatal cows. Their whole blood was collected from the tail vein every5d to detect the activity of serous GSH-PX, SOD, GSH and the content of CAT, MDA by double antibody sandwich ELISA method. Mn, Zn, Cu, Se, Fe and other trace elements were detected by ICP-MS.Results showed that the serous GSH-Px activity in perinatal cows reduced gradually until the childbirth. Serous SOD activity rose gradually from prenatal45d to prenatal15d, thereafter declined, and showed a lower level in the day of childbirth. Serous CAT activity began to rise on prenatal20d, but decline from prenatal10d. It downed to the bottomed on the calving day, thereafter showed a trend of elevation. The content of serous GSH was on the rise in antenatal; it reached to the peak on the calving day, and then declined gradually. The content of serous MDA began to rise gradually on prenatal30d; it reached to the peak on prenatal5d. In addition, there were significant differences (P<0.05) between different times. There was a significant or extremely significant positive correlation (P<0.05or P<0.01), comparing MDA with GSH and CAT, respectively. The content of serous Mn increased before the childbirth, postpartum level was higher than the prenatal one generally. The content of serous zinc showed a trend of decrease in the antenatal, reaching to the bottom on the calving day, then increased gradually. The content of serous Cu didn’t show an obvious change in prenatal, it moderately elevated from the calving day (0d) to postnatal10day. The content of serous Se started a trend of increase in prenatal20d, reaching the peak on the calving day and postpartum5d, thereafter reduced gradually and maintained at a normal level. The content of serous Fe maintained fluctuations in prenatal and showed a significantly reduction on the day of calving. In addition, there were significant differences (P0.05) between the trace elements of different times. There was a significant or highly significant negative correlation(P<0.01or P<0.05), comparing serous selenium and GSH-Px activity, serous Cu content with SOD activity, respectively. All results above indicated that there was imbalance of oxidation state in perinatal cows, and change of dynamic balance of the serous trace elements.