Related Research On The Correlation Between Phenotypic Character And Different Cytoplasm In Sugarcane (Saccharum Officinarum L.)

Sugarcane is the most important sugar and energy crops worldwide.At present, selective breeding of sugarcane crossing hybrid combination mainly develop the potentiality of nuclear gene to sugarcane variety, but the excavation and utilization of sugarcane cytoplasm genome and relate facourable cytoplasm genome is blankness yet. So the plasmagene of sugarcane varieties all over the world limited in Bandjarmasin Hitan, Black Cheribon, Badila and Kaludia Boothan, makes some of the main is controlled by cytoplasmic genes or karyoplasm interaction effect of strain improvement blindly and inefficient. In order to demonstrate the correlation between parents of different sugarcane plasmagene and major genetic phenotypic character, karyo DNA used to authenticate descendants and differential gene expression was analysed by the means of phenotype identify and genetic polymorphisms of cpDNA and mtDNA based on the pilot studies about different cytoplasm parents and phenotype of their offspring, reciprocal cross was conducted in parents with different plasmagene. The foundation for excavating and using plasmagene in sugarcane breeding would be laied. The main results are obtained as follows:1.18SSR primer pairs authenticated640descendants of material, true hybrid rate is higher than90%of the three combination is47,92, and68combinations which of their maternal cytoplasmic sources are Black CHeribon. Others true hybrid rate less than70%of3combinations is102,135and107, there are two combinations of maternal cytoplasmic source for Bandjarmasim Hitam, but one’s Kaludia Boothan. 2. Using18640descendants of SSR primers identification material, results show that the three true hybrid rate is higher than90%portfolio combination of47,92, and68combinations of maternal cytoplasmic sources are Black CHeribon. In true hybrid rate less than70%of three portfolio combination of102and135and107, there are two combinations of maternal cytoplasmic source for Bandjarmasim Hitam, a combination of the source of cytoplasm Kaludia Boothan.3. Improved DNase I differential centrifugation processing method is used to extract sugarcane chloroplast DNA, can be used for subsequent sugarcane chloroplast gene at the molecular level.Chloroplast trnL gene can be used to cut sugarcane varieties, hand close identification and its allied genera.4. For volunteers to four different cytoplasmic source material equipping reciprocal cross combinations progeny t inspection, found that volunteers’ combination of plant height, stem diameter and effective, have no obvious difference,135, and138combinations of chlorophyll content difference reached significant level, but they have the same source of female parent cytoplasm, are Bandjarmasim Hitam, illustrate the above properties is mainly controlled by nuclear genes, less affected by the reciprocal cross.5. Smut incidence in515and481,514and473were28.4and18.8,28.4and18.8, reciprocal cross between t test confirmed P=0.00636and P=0.00781, both difference between the reciprocal cross combinations reached extremely significant level, and the cytoplasm of reciprocal cross combinations of parent source of same, Badila and Bandjarmasim Hitam, when with the cytoplasm source for Bandjarmasim Hitam ROC22different parent material and ROC10as maternal, child smut incidence is very close to,28.4%and28.8%, respectively, when the source in the cytoplasm of Badila yacheng58-47as the female parent and ROC22and ROC10respectively with reciprocal cross combinations, offspring of smut incidence almost unanimously,18.8and18.9respectively.