Mirror Carp Scale Was Development Related Gene Cloing And Analysis Analysis Of (FGF1a/FGFR1a)

Through specific fish and scale development related gene, FGF/FGFR, EDA/EDAR, TPT1gene.Choose from the fgf1a/fgfr1a of the gene to study.Fibroblast growth factor (FGF) is an important growth factor plays an important role in cell division,growth and differentiation processes. We use laboratory carp EST database design RT-PCR primers havebeen part of the fragment FGF1a gene-specific primers and then use the EST database design laboratorycarp CDS contains full-length, full-length FGF1a genes obtained CDS. After cloning and sequencing, wefound that1. A full-length mirror carp FGF1a CDS gene comprises444bp, which encodes a146amino acidprotein containing a receptor binding site.2.Sequence alignments with other species results showed that carp and zebrafish protein similarityFGF1a highest, reaching85.03%, while its distantly related human, mouse, and chicken toad Xenopuslow similarity, namely49.68%,49.68%,44.59%,45.22%, overall, the protein conserved amongdifferent species is still relatively high.3. Organizations RT-PCR results showed that, FGF1a genes in carp heart, kidney, gills, muscle, liverand skin were expressed, but the expression is particularly evident in the skin.4. According to the gene sequences of probes obtained by in situ hybridization gene expression wefound FGF1a higher scales on the skin of the fish just long, indicating that the gene may be involved in thedevelopment of scales; while the caudal, dorsal and ventral fins also have specific expression of thesignal, indicating FGF1a may also be involved during development rays.5. The full-length gene carp FGFR1a CDS contains2421bp, which encodes a813amino acidprotein. Overall, the protein is conserved among different species is still relatively high.6. RT-PCR by various organizations showed, FGFR1a carp genes were expressed in the heart,kidneys, gills, muscle, liver and skin.7. According to the gene sequences of probes obtained by in situ hybridization found FGFR1a gene expression at6hours after the beginning of fertilized embryos,12hours appeared strongly expressedspecifically, after expressing waning. FGFR1a gene expression in the adult fish just long scaly skin higher,indicating that the gene may be involved in the development of scales; while there has also been specificexpression signal in caudal, dorsal and pelvic fins, the description FGFR1a rays may also be involved inthe development of process.