Parentage Identification Of Heilongjiang Wild Carp And The German Mirror Carp Cross Combinations On M Icrosatellite DNA Markers And Carp Immune Function Gene Snp Research

A set of8microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp (?)×German mirror carp(?), and inverse cross group of the German mirror carp(?)×Heilongjiang carp(?). The results showed that:(1) observed heterozygosity values of parents and offsprings were0.6071,0.7143,0.6818and0.7083,0.5386,0.7438, and polymorphism information content were0.4421,0.2813,0.5535and0.6735,0.3759,0.6241in two groups, indicating that all8microsatellite loci showed polymorphism.(2) In the obverse cross group, the accumulative exclusion rate using8microsatellite loci was99.92%and the appraisal ability was98.00%while confidence was95%. In the inverse cross group, the accumulative exclusion rate was99.99%and the appraisal ability was99.00%.(3) about relation between microsatellite markers and appraisal ability, we compared the accumulative exclusion rates and the appraisal ability using3,4,5,6,7,8microsatellite markers.6and8high polymorphism markers were successfully applied in paternity deter mination of two cross groups. The study of carp immune function genes showed that:(1) four of the11carp immune function genes had the SNP. TLR2, TLR3a TLR3b, TLR7a, TLR7b, I type of IFN, and TRAF6had no SNPs after SSCP detection in292individuals of8carp populations, but IL-1β, TLR9, MyD88a, MyD88b gene polymorphism were higher.(2) about IL-1β gene,13variable sites was detected in IL-1β gene of292individuals in eight carp groups, including three non-synonymous mutations. NJ individuals clustering of eight groups concluded that most of the Songpu mirror carp, the Songhe carp and Heilongjiang wild carp individuals first got together, followed by the German mirror carp survival group and the Songpu carp, German mirror carp death group and then the Yibu carp, the Datou carp together. The results of population cluster analysis according to genetic distance was roughly the same as the individual cluster analysis.(3)TLR9gene detected37variable sites in292individuals, of which20variable sites caused amino acid change. The frequency distribution of C.988> T, c.1237T> C, c.1700T> A, c.1770A> G, c.2278T> C, c.2307T> C, c.2377A> G, c.2547A> T, c.2580T> A, c.2646T> C in eight carp groups were the highest, which c.2580T> A and c.2646T> C were distributed in every carp populations.(4) Six variable sites were detected in the intron regions of MyD88a gene. Nine variants were detected in MyD88b gene, a non-synonymous mutations.