Molecule Identification And Analyse Of The Symbiont Microorganism In Mealybug (Phenacoccus Solenopsis Tinsley)

The cotton mealybug Phenacoccus solenopsis (Tinsley)(Hemiptera:Pseudo--coccidae) was described originally from the USA in1898. It remains as a mi nor pest for nearly one hundred years. Phenacoccus solenopsis has emerged as major pest of cotton since it invaded India, Pakistan and China. This exotic mealybug caused huge economic loss and has been the current topic of researc h. This pest is a sap sucking insect and rely on the phloem sap of the plant. There are many kinds of microorganism in vitro and vivo, including symbiotic bacteria and fungi. The primary symbiont provide essential amino acids to thi s pest which are obbligato to maintain its’normal life. The secondary symbion t which are not necessary for the pest plays an important role in contributing to the flexibility, the enemy resistance and the competition between other inse ct of the host. The current mealybug control most by chemical methods and th is must lead to the environmental pollution, the break of biodiversity, the drug resistance of the mealybug and many other issues. However, as a environment al friendly way to control the spread of mealybug, fungi fascinate more and m ore attentions in recent years as a biocontrol way. By sequencing variable V4region of16S rDNA of this mealybug employing Illumina MiSeq platform, we analyzed the diversity of symbiotic bacteria in the cotton mealybug, meanwhil e, five kinds of fungi were isolated from the adult female mealybug by culture medium with chlorampheniclo. And the result were as follows:(1) The16S rDNA sequencing analyses indicate that the mealybug hold many kinds of symbiotic, most are Proteobacteria, Firmicutes and Bacteroidetes. And on phylum the dominate Proteobacteria isn’t different significant in female and male mealybug, while the others tend to be different significant. On genus, most of the symbiotic are different significant between the female and male mealybug.(2) The fungi displayed that the Ascomycota.sp strain E-Yming R possess the highest infection rate in adult mealybug which reach up to66.67%. However, the Colletotrichum gloeosporioides strain I-Yming R manifest the lowest rate that is deep as3.33%. The multiple infection analyse shows that this pest is apt to hold several kinds of fungi but it isn’t tend to be the more the better. This may owe to the interaction between the different fungi in mealybug, and thus makes a dynamic equilibriu in the mealybug.