Effect Of Symbiotic Microbiota In Honeydew And Gut Of Phenacoccus Solenopsis Tinsley (Hemiptera:Pseudococcidae) Under Stress Of Natural Enemy

Mutualistic relationship between ants and honeydew-producing insects(e.g.mealybugs and aphids)is a common inter-species relationship in ecosystems and an important mechanism for maintaining the structure and function of ecosystems.In order to study the role of symbiotic bacteria in regulating the reciprocal relationship between Solenopsis invicta Buren and Phenacoccus solenopsis Tinsley,the changes of symbiotic microbiota of P.solenopsis natural enemy stress were studied;the culturable microorganisms in the body and honeydew of P.solenopsis were isolated and purified;and the volatile components of the honeydew of P.solenopsis were identified.The main results are as follows: 1.Changes of symbiotic microbes in the intestines and honeydew of mealybug under the stress of natural enemiesThe high-throughput sequencing method was used to determine the diversity of symbiotic microbes in the intestines and honeydew of mealybug.At the door classification level,Proteobacteria,Actinobacteria,Firmicutes,Firmicutes,Cyanobacteria,Planctomycetes,Deinococcus-Thermus,Chlamydiae,Verrucomicrobia and Acidobacteria are the top 10 species of abundance,and Proteobacteria,Actinobacteria and Firmicutes are the dominant species for various treatments.Through differential analysis,the relative abundance of Sphingomonas in the intestinal tract was significantly higher than that of the other two stresses after 48 h of wasting stress.The relative abundance of Staphylococcus in the intestinal tract was significantly lower than the other two.2.Isolation,purification and identification of cultured bacteria from mealybug’s gut and honeydew.Four kinds of bacterial culture medium were used to separate the cultivating bacteria of P.solenopsis Tinsley and honeydew.The 16 S r DNA of bacteria could be cultured by PCR and compared by BLAST.The results showed that Paenibacillus and Microbacterium were separated from honeydew.isolated from the genus Arthropod,belonging to Arthrobacter,Myroides,Staphylococcus,Brevudimonas,Sphingomonas Novosphingobium,Brevibacillus,Enterococcus,Acetobacter,Micrococcus,Paenibacillus,Curtobacterium,Microbacterium,Brachybacterium,Bacillus were separated from P.solenopsis.3.Analysis of volatile organic components(VOCs)in honeydewThe gas chromatography and mass spectrometry(GC-MS)were used to identify the volatile components in the honeydew,and various volatile organic compounds such as ketones,aldehydes and olefins were identified from the honeydew.