The Effects Of Manganese-induced On MRNA Expressions Of HSPs In Chicken Spleen Lymphocytes In Vitro

Manganese (Mn) is an essential trace element, which is distribution in many organs and tissues of biological organism, and plays an important role for maintain basic life activities and main physiological function of organism. However, when organism exposure to excessive Mn or prolonged exposure to Mn can lead to pathological damage and produce toxic to the body. In recent years, with the development of the immune technology, the research about Mn-induced immune toxicity has drawn more and more attention. So far, the domestic and foreign researches about Mn toxicity focus on nervous system and genital system in mammals and aquatic animals, while the reports about immune system were seldom, even of poultry immune cells. The purpose of this study was to investigate the effect of Mn-induced cytotoxicity on heat shock proteins in chicken spleen lymphocytes. Lymphocytes were cultured in medium in the absence and presence of manganese chloride (MnCl2)(2x10-4,4×10-4,6×10-4,8×10-4,10×10-4, and12x10-4mmol/L) for12,24,36, and48h in vitro. Then, the mRNA levels of HSP27, HSP40, HSP60, HSP70, and HSP90were examined by real-time quantitative PCR. Draw the following conclusions:(1) The mRNA expression of the heat shock proteins was induced at lower concentrations of Mn.(2) The mRNA expression of the heat shock proteins was inhibited at higher concentrations of Mn.(3) Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90mRNA expression in chicken spleen lymphocytes in vitro.