Tillering And Yield Associated Genes Cloning And Expression Characteristics Research In Gramineous Forage Grass

Tillering is a process which is independent from the main branches. It occurs at the stemof gramineous plants and determines the spike number of crop. The pasture, weed and otherclonal plants with saline tolerant, barren tolerant, frigid tolerant and other superiorperformances, which were cloned by tillering, become to the dominant species. In here, wecloned the corresponding functional genes of Leymus and Festuca using the rice functionalgenes as reference. The obtained corresponding functional genes were analyzedcomprehensively and verified. The developmental stage and tissue specificity data of thecorresponding functional genes were detected by real-time fluorescent quantitative PCR.Combining with the comprehensive analysis of these functional genes, it reveals the influenceof grass morphological differentiation and has contribution to the yield. Moreover, theapplication prospects of these genes have been preliminarily evaluated.Six functional genes of Leymus and Festuca were obtained through the electronic clone,and submited to Genbank to get accession numbers. In Festuca, the accession number ofOsMdp1which encodes a MADS-box transcription factor is JQ317021, and the accessionnumber of OsHAP3which controls the chlorophyll synthesis is JX312558. In Leymus, theaccession number of DW2gene which encodes an annular E3ubiquitin ligase is JQ083606,the accession number of cca-bbp gene which codes the chlorophyll a-b binding protein isJX397992, the accession number of TB1gene which codes regulation lateral bud growth ofnegative regulation factors is KC479014, the accession number of PROG1gene which codeszinc finger transcription factor is KC493770. For ACC gene, its conserved sequence wasobtained through homology cloning technology, and the sequence was341bp and thesimilarity with L. chinensis LcA04F05gene is65.29%. By bioinformatics tools, the predictedopen reading frame of the gene encodes99amino acids, and molecular weight is11,275 Dalton. The gene coding region is located41-341bp and it is hydrophilic protein. The aminoacid sequence encoded frame between the translated amino acid sequence of the geneencoding wheat ACC for protein homology analysis have47.83%consistency, andconsistency conserved region of up to99%. The secondary structure with no other secondarystructures is composed of α-helix (representing37.00%), β-fold (accounting for18.00%),random coil (representing45.00%). It belongs to α-ketoglutaric acid/iron-dependentdioxygenase (2-oxoglutarate/Fe(II)-dependent dioxygenase) proteins family. This workprovided research foundation about saline resistance related regulation mechanism inLeymus.LRC1gene expression in Leymus was investigated by the influence of illumination time.From the research results of leaves and leaf sheaths parts, the expression quantity of LRC1gene was influenced by illumination time. Detection results of the stem portion indicated thatthe gene expression quantity of LRC1showed a trend of gradual decline under normaltraining or reduced illumination time. But the extent of decline of the former was slower thanthe latter. As time goes on and with the advancement of differentiation, the decrease trend ofgene expression quantity fluctuated in the late stage of sampling. New function may beperformed by this gene in the organization part. The detection results of the combinationbetween stem and root speculate that the decrease of photoperiod have inhibition toexpression of LRC1gene. And under the condition of applying process, LCR1geneexpression showed a trend of gradual decline. It suggested that the gene in this part of theorganization may exercise the new functionality. Test results combined with the root portionof the stem speculation shorter photoperiod genes on LRC1inhibit gene expression, but notapplied to the treatment conditions LRC1gene expression levels fluctuate under the premiseof showing the expression of basic steady trend speculated that maintaining substantiallyconstant the amount of gene expression in normal culture conditions. Our research provides ademonstration for the study of other environmental factors affecting the gene expression, aswell as lays the foundation of the expression and regulation of gene function mode in otherGramineae species. This study provides the demonstration for other related research. Potential application isthat: on the one hand, it provides experimental basis for cultivating good grassland pasturespecies and planting artificial grassland. On the other hand, it provides guidance to improvethe production of natural and artificial grass.