Molecular Cloning And Functional Study Of Kruppel-homolog1(Kr-h1) From Nilaparvata Lugens(Stal)

Juvenile hormone(JH) and molting hormone(ecdysone) were very important hormone to mainly be regulated during the lavare, pupa and adult stage. There are seven kinds of JHs, however the molecular mechanism of JH signaling is poorly understood. Recent studies showed that JH modulates or suppresses ecdysone signal during the insect development and metamorphosis. Kruppel-homolog1is a transcription factor containing C2H2-type zinc-finger motifs, which is downstream of JH receptor Met. Kr-hl is a key player in the JH. Also kr-h1mainly has the function of regulating the larval growth, and in some species there were other functions such as strongly associated with the foraging behavior of honey bees, related to the formation of Drosophila melanogastr neuron and so on, thus kr-h1may functions widely and depletion of which could form a variety of phenotypes.We cloned kr-h1from Nilaparvata lugens by RACE and reverse transcriptase PCR, studied the expression profile by qPCR and studied it’s function by RNAi and RNA-seq. The results showed that the expression of kr-h1mRNA levels were high at the embryonic stage, remained at a moderate level in the larval-propupal stage, low in the pupal stage. The expression of kr-h1is sensitive to hormonal treatment especially during the demarcation point of larval. Through RNA interference, it is obviously leads to phenotype in wing and genitals, etc. In addition, through analyzing the expression profile changes of the genes and later confiemed by Real Time PCR(e.g. cuticular protein), we found the potential function of kr-h1. Kr-h1is crucial transcription factor in terms of JH actions, to obtain the preliminary characteristics of this gene should help us understand the molecular mechanisms of JH, as well as benefit to finding better approach to protect our rice production.