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Defense Behavior Of Pinus Sylvestris Var.Mongolica To Cone Pests And The Research Of Cone Pest Control Techniques

Posted on:2015-10-18Degree:MasterType:Thesis
Country:ChinaCandidate:Q S ZhangFull Text:PDF
GTID:2283330434451194Subject:Forest Protection
Abstract/Summary:PDF Full Text Request
This paper took Pinus sylvestris var. mongolica as object for the study. The defense behavior of Pinus sylvestris var. mongolica for pest damage and the control of cone and seed pest were studied. To the damage, it responsed in many aspects, such as nutrients, enzymes and other defenses material.As the pest damage to deepen, the water content of the cones decreased which drop22.02%, even cause the cones discoloration. The content of total soluble sugar which cannot be absorbed directly is on a rise in26.90%, and the content of reducing sugar which cannot be absorbed directly is in a drop in40.75%. The ratio of Sugar/protein has also changed. It fell after rise in the cones damaged by Dioryctria mutatella Fuchs and always rise in the cones damaged by Pissodes validirostris Gyll. Studies have shown that the ratio of sugar/protein rise will increase aging larval mortality. Upon seed pest harm, the reactive oxygen species in the trees increased. And then, three defensive enzyme SOD, POD, CAT in the cones began to play a role. At the beginning of the hazard, the SOD activity strengthened, from97.41±6.58U/g to154.94±3.50U/g,and it will start to decline with the constantly feeding damage of seed pests, till120.08±6.41U/g. As different, the activity of CAT and POD are in a clear downward trend. From321.67±89.17U/g and255.60±17.67U/g.min down to4.20±0.42U/g and61.80±1.59U/g.min respectively. Hazard initially, tannins and flavonoids in the cones are increased to discourage insect feeding, affect its nutrient absorption. It increases from11.5mg/g and20.5mg/g to14.8mg/g and24.1mg/g respectively. The flavonoids will reduce later. Minimum of only15.7mg/g.In this paper, both chemical and biological methods were used to control cone pests. Spray method was used to control the adults of Pissodes validirostris Gyll. Determination by toxicity testing, filter out1%-emamectin benzoate emulsifiable concentrate diluted for1500times,3%-ragweed microcapsule diluted for800times and2.5%-veratridine microcapsule diluted for500times for the the wild caged combat.3%-ragweed microcapsule diluted for800times works best, corrected mortality is88.24%after72h.30%-acephate,40%-omethoate and5%-imidacloprid were used to control the larvas of Pissodes validirostris Gyll by trunk coating method. They all have certain control effect, and no peeling treatment set significantly lower than the skinning group.40%-omethoate diluted for5times peeling treatment did best, corrected mortality is70.69%. Artificial overwintering protection can significantly improve Scambus punctatus survival. Artificial release Scambus pumctatus can significantly improve its parasitism rates. Scattered releasing is better than stacking. Therefore it can be concluded that, using3%-ragweed microcapsule diluted for800times by spray method,40%-omethoate diluted for5times by trunk coating after peeling method and using Scambus punctatus biological control Pissodes validirostris Gyll are feasible.
Keywords/Search Tags:Pinus sylvestris var. mongolica, cone and seed pests, defensive substance, chemical control, biological control
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