The Functional Identification Of A Poplar Unknown Gene Potri.016G101900

The secondary growth of Plants is the process that vascular cambium constantly produce new secondary xylem tissue and secondary phloem tissue. Research suggests that hormones, transcription factors, regulatory factors are all involved in the regulation of secondary growth for many years.But the molecular basis of secondary growth is still quite weak. Identified to participate in important molecules and their role in the process of secondary growth of forest, is one of the important theoretical basis for molecular breeding modified trees. This study explores the function of an unknown Populus trichocarpa gene Potri.016G101900,we found that it may participate in influence the secondary growth of poplar. The main research results are as follows:The Potri.016G101900of Populus trichocarpa which full length of coding sequence the gene sequence contains300bp CDS,100amino acids. Homology search showed that different plants such as arabidopsis, rice and maize species have the homologous genes; Sequence comparison showed that the speculation of the gene encoding protein amino acids in different plant species exist in the more conservative. A semi-quantitative RT-PCR analysis showed Potri.016G101900genes of Populus trichocarpa has the transcriptional expression in the tissues and organs of root、stem and leaf; the expression level of internodes1to6,9increased significantly with the level of Stem section lignification degree. This result suggests that the gene may be involved in secondary growth of Populus trichocarpa.Fusion of green fluorescent protein (GFP) strategy, construct pGWB5-Potri.016G101900plant expression vector, excessive expression Potri.016G101900-GFP in arabidopsis thaliana; Laser confocal microscopy analysis showed that in transgenic arabidopsis thaliana hypocotyl and leaf tissue cells spontaneously and chlorophyll fluorescence green fluorescence signal coincide, suggests Potri.016G101900-GFP is likely to locate in the chloroplast. Similarly, in transgenic arabidopsis root green fluorescent signal area is likely to be plasmids.Through transformation Potri.016G101900of arabidopsis which combined with chemical staining analysis showed that overexpression of Potri.016G101900arabidopsis thaliana appear anomalous cell shape in radicle, secondary metabolites deposite,lignin ectopic accumulation. In addition, in the transgenic arabidopsis thaliana which overexpress the Potri.016G101900also resulted in the content of lignin increased in the area between interfascicular region and xylem. These results suggest that Potri.016G101900genes is involved in secondary growth of Populus trichocarpa.In addition, in order to identify the function of Populus trichocarpa Potri.016G101900in reverse genetics, we build amiRNA-Potri.016G101900carrier and express it in the84K poplar, obtained transgenic poplar plant which the transcriptional expression of Potri.016G101900is silence. This provides the genetic material in order to identify the function in poplar secondary growth of the unknown gene.