The Functional Analysis Of A Poplar Cyclase Gene Potri.006G237100

Cyclase is a enzyme of catalysis nucleoside three phosphoric acid to produce cyclic nucleotides, widely exists in animal, plant and microorganism. There are many kinds of genes present in the plant, which are involved in many biological functions of plant. In the study, a kind of cyclic enzyme of rice was involved in the stress by controlling the level of reactive oxygen species. Tomato red element epsilon cyclase gene overexpression is associated with photosynthesis, tomato red pigment beta cyclase gene involved in wheat beta carotene biosynthesis. The gene involved in the salt tolerance of the crop, and the excessive expression of this gene increased the biomass of rice under the environment of high salinity. However, the information or function of the cyclase gene in trees is poorly reported. In the previous study, we isolated a poplar tree ring gene Potri.006G237100, which may be involved in wood formation. In order to identify the function of this gene, the main results obtained are as follows:The full-length CDS sequence of the Potri.006G237100 coding region contains 507 nucleotides, encoding 169 amino acids. The homology search revealed that there were homologous genes in different plant species such as Arabidopsis thaliana, soybean and grape. Sequence comparison showed that the gene encoding amino acid protein are higher in different plant species. Semi quantitative RT-PCR analysis showed that fruit Mao Yang cyclization enzyme gene Potri.006G237100 in xylem, petiole and root tissue in high expression abundance, and the transcriptional level and woody stems of synchronization.Construction of plant vector Potri.006G237100-GFP fusion. In Arabidopsis excessive expression Potri.006G237100-GFP, laser confocal microscopy for detection of transgenic Arabidopsis root in GFP fluorescence signal. The results showed that signal is concentrated in the cytoplasm, in the periplasmic region also has a small amount of signal distribution, indicating that Potri.006G237100 protein might be localized in the cytoplasm. By transformation of Arabidopsis thaliana obtained Potri.006G237100 overexpression of genetic material, tissue anatomy combined with chemical staining analysis showed, and wild type compared to the over expression of irregular xylem cells Potri.006G237100 gene in Arabidopsis thaliana, interfascicular cell wall thickening; overexpression Potri.006G237100 gene of Arabidopsis thaliana root length and wild type Arabidopsis root looks than the variable length. These results indicate that the cyclase gene Potri.006G237100 may be involved in the formation of poplar wood (secondary cell wall).