Study On Tanshinone Breast Implantation’s Antimicrobial Effect Of Common Mastitis Pathogens And Clinical Efficacy

Objective: Mastitis is costly, harmful and hard to prevent. It hinder the development ofthe dairy industry worldwide, or even threats human beings. Antibiotics and chemical drugsare still first choice for treatment of mastitis in dairy cows, however, antibiotic resistance anddrug residues have become new problems. Traditional Chinese medicine is natural, almost noresidue, and no drug resistance, which become a unique advantage in the treatment of mastitisin dairy cows. Salvia is commonly used in clinical traditional Chinese medicine, with itsfunction of activating blood circulation to dissipate blood stasis, passing through the pain,cooling carbuncle, etc. This research aims to study the antimicrobial effect and clinicalefficacy of Salvia extracted Tanshinone based medicine-"Tanshinone breast implantation "against common mastitis pathogens.Method:(1) using MacConkey tablet isolated Escherichia coli, using manitol salt agarisolated Staphylococcus aureus, using the CAMP test isolated and identified Streptococcusagalactiae, and identified these bacteria by microscope and biochemical tests.(2) using thebroth dilution method determined and compared the minimum inhibitory concentration (MIC)and minimum bactericidal concentration (MBC) of " Tanshinone breast implantation" andcontrol drug "Shuangding injection".(3)40cows suffering from clinical mastitis, divided intofour groups:10for Tanshinone high-dose group (46g/d,7d),10for Tanshinone recommenddose group (23g/d,7d),10for Tanshinone low dose (11.5g/d,7d),10for Shuangding group(10mL/d,7d). Study the clinical efficacy by counting the milk somatic cell count (SCC) andcounting its cure rate.Results:(1) Escherichia coli, Streptococcus agalactiae and Staphylococcus aureus wereisolated;(2) MIC of " Tanshinone breast implantation " for Escherichia coli, Streptococcusagalactiae and Staphylococcus aureus were62.5mg/mL,31.25mg/mL,15.63mg/mL;MBC were of62.5mg/mL,31.25mg/mL,31.25mg/mL; MIC of "Shuangding injection" forEscherichia coli, Streptococcus agalactiae and Staphylococcus aureus were62.5mg/mL,31.25mg/mL,31.25mg/mL, MBC were of62.5mg/mL,62.5mg/mL,62.5mg/mL.(3) SCC:The difference between Tanshinone breast implantation high dose group and recommend dosegroup was not significant (P>0.05), the difference between Tanshinone breast implantation agent high-dose group and low dose group was significant (P <0.01), the difference betweenTanshinone breast implantation agent recommend dose and Shuangding injection group wassignificant (P <0.05). The cure rate: The difference between Tanshinone breast implantationhigh dose group, recommend dose group and Shuangding injection group was not significant(P>0.05); The difference between Tanshinone breast implantation high dose group,recommend dose group and low group was significant (P <0.05).Conclusion:(1) Escherichia coli(E. coli), Streptococcus agalactiae (S. agalactiae) andStaphylococcus aureus (S. aureus) were isolated.(2) Tanshinone breast implantation’santimicrobial effect is totally better than Shuangding injection.(3) Tanshinone breastimplantation is more effective than Shuangdinginjection. The recommend dose,23g each day,continuous infusion7d, have a good treatment effect.