Isolation, Identification And Antibiotic Susceptibility Test Of Pseudomonas Aeruginosa Isolated From Zebra

Pseudomonas aeruginosa (P. aeruginosa) is a primary or opportunistic pathogen inhumans and animals, and is a common clinical pathogen. There is increasing evidence of P.aeruginosa infections with the gradual development of aquaculture. Infections of P.aeruginosa have been reported in the crested ibis, red pandas, mink, bamboo rat, foxes andother wild animals. The present study on separation in a fatality zebra suspected strains of P.aeruginosa were isolated and identified. The antibiotic susceptibility of isolates was alsoevaluated. The infection of P. aeruginosa was initially identified by clinical observation andgross necropsy, and then the isolated strains were further identified by in vitro culture, PCRidentification and16S rRNA sequencing, as well as drug sensitive test by K-B paper diskdiffusion method. The results showed that smooth, slightly uplift, edge neat dark greycolonies were observed after inoculation of samples, including pericardial fluid, pleuraleffusion, pulmonary, lung and liver tissue fluid, to the ordinary agar plate and culturedovernight. Gram staining microscopic examination showed the isolates were gram negativebacteria. After incubation of P. aeruginosa in ordinary broth tubes for24h, broth culturemedium showed evenly-turbid, appearance from pale yellow to yellowish green. Appearanceof green upper liquid of broth culture medium disappeared after gently shake, and thenrecovered to green appearance after stewing. Precipitation of mucus was also observed at thebottom of the tube broth. PCR amplification and16S rRNA sequencing analysis furtherdemonstrated that the homology of isolates with P. aeruginosa from GenBank more than99%.Analysis of genetic evolution revealed that isolated strains are region specific. The drugsensitive test revealed that the isolated P. aeruginosa have strong sensitivity to the thirdgeneration quinolone antibacterial drugs, including norfloxacin and ciprofloxacin, and havemedium sensitivity to streptomycin and pefloxacin, and is resistance to neomycin,doxycycline, kanamycin, ceftriaxone, ampicillin, rifampicin, clindamycin, cefazolin,trimethoprim sulfamethoxazole, and erythromycin.In conclusion, the pathogen isolated from a fatality zebra is identified as P. aeruginosaby using clinical diagnosis and laboratory diagnosis. The isolated P. aeruginosa have strong sensitivity to the third generation quinolone antibacterial drugs, and have medium sensitivityto streptomycin and pefloxacin, and is resistance to neomycin, doxycycline, and so on. Thepresent study provides an experimental basis for the reasonable application of antibiotics andclinical diagnosis of P. aeruginosa infections in zebra.