Experimental Study On The Effect Of Mahonia And Ligustrum Lucidum On The Immune Functional Of Lung-oi Deficiency Mice

Deficiency of Lung-Qi in animal model is the basis ofexperimental studies of pulmonary diseases. In this study, modeling methodsofLung-Qi Deficiency Syndrome animal model using mice as experime ntal animals,immune functional change of Lung-Qi Deficiency mice model as well as theeffect of Ligustrum lucidum and Mahoni a on the immu ne function of Lung-QiDeficiency mice were studied. Now the main research content and results aresummarized as follows：1． Deficiency of Lung-Qi in Balb/c mice model were establishedrespectively by single SO2, SO2with chill factor stimulating method, singlecigarettes smoked and cigarettes smokedwith chill factor stimulating method;Deficiency of Lung-Qi in KM mice model was established by cigarettessmokedwith chill factor stimulating method. The control groups of two strains ofmice were set up respectively. The indexes such as body weight, organ index,necropsy and histopathological changes in the lungs were detected aftermodeling. The results suggested that cigarette smokedwith chill factorstimulation was superior to other three methods, and compared with KM mice,Balb/c mice are the preferred animal in Deficiency of Lung-Qi animal model.2． Deficiency of Lung-Qi in Balb/c mice model was established bycigarettes Smokedwith chill factor stimulation. The immunity indexes such asactivity of the peritoneal macrophage, the proliferation of lymphocytes, theantibody productionsecretedby B-cell in vitro, the quantity of IL-2, IL-4, IL-6,IL-10, IFN-γ, TNF-α, NO in serum and sIgA, sIgM in lung tissue homogenatewere detected. The results showed thatcompared to the control group， theactivity of peritoneal macrophages in Lung-qi deficiency model mice decreasedsignificantly (P<0.01)； proliferation of T and B lymphocytes increasedsignificantly (P<0.05)； Cytokine IL-2increased significantly(P<0.01)； thequantity of IFN-γ increased significantly (P<0.05); the quantity of IL-6decreased significantly (P<0.01); the quantity of IL-4increased significantly at(P<0.05); the quantity of NO decreased significantly (P<0.05); the quantity ofsIgA and sIgM increased significantly (P<0.01). So the immunity index testresults showed that the immunological function of Deficiency of Lung-Qi in Balb/c mice was significantly damaged.3. Each group of animals were fed high dose, middle dose and low dose ofLigustrum lucidum water de coction and Mahonia water decoctionrespectively byintragastric feeding or intraperitoneal injection. The immunity indexes such asactivity of the peritoneal macrophage, the quantity of haemolysin in serum weredetected3days later. The results showed that intragastric feeding was betterway than intraperitoneal injection; Ligustrum lucidum water decoction andMahonia water decoction could improve the activity of the peritonealmacrophage and the quantity of haemolysin in serum, and the optimal effectivedosages were both20mg/g.4. Deficiency of Lung-Qi in Balb/c mice model was established bycigarettes smoked with chill factor stimulating method. After the end ofadministration, each group of animals were fed Ligustrum lucidum WaterDecoction and Mahonia Water Decoction by intragastric feeding for7days. Theimmunity indexes such as the activity of the peritoneal macrophage, thelymphocyte proliferation, the quantity of IL-2, IL-4, IL-6, IFN-γ, NO in serumand sIgA, sIgM in lung tissue homogenate were detected. The results showedthat Ligustrum lucidum Water Decoction and Mahonia Water Decoction couldincreasethe activity of the peritoneal macrophage and the quantity of IL-2, NOin serum, also could decrease the lymphocyte proliferation, the quantity of IL-2,IL-4, IFN-γ in serum and sIgA, sIgM in lung tissue. In conclusion, Ligustru mlucidum Water Decoction and Mahonia Water Decoction have up-regulateimmunofunction of Lung-Qi Deficiency mice model, and the optimal effectivedosages were respectively20mg/gof Mahonia Water Decoction,15mg/gofLigustrum lucidum Water Decoction.