Effect Of Two Chitosans On Growth Performance And Immunological Function Of Mice

The 224 Healthy equal male and female JCR mice were chosen to measure the molecular weight and deacelylated degree of two chitosan , the effects of body weight gain and immunological function on mice.The 224 mice were randomly divided into eight groups,each with 28 mice.The 8 treatments were basic diet adding 100mg No 1 chitosan,basic diet adding 200mg No 1 chitosan,basic diet adding 500mg No 1 chitosan,basic diet adding 100mg No 2 chitosan,basic diet adding 200mg No 2 chitosan,basic diet adding 500mg No 2 chitosan,basic diet adding 100mg oxytetracycline and control.The rate of T-lymphocytes,the phagocytic indexes of intraperitoneal phagocyte and the determination of serum lysozyme and protection rate of infectious individuals were measured.The results showed:1. The [η] values of two chitosans were 121.4,161.7,the molecular weight of the two chitosans were 7.03×104,9.72×104,the D.D% of two chitosans were 83%,63%.2. The average daily food intake of evry trial group increased by 6.31% 7.24%, 3.34%, 3.90%, 4.08%, 2.05%, 7.05% compared to the control group,but there were no significant differences among the 8 groups(P > 0.05);About the average daily gain,the 1, 2, 3, 4, 5, 6 groups increased by 4.99%,5.32%,4.83%,1.50%,1.16%,0.67% compared the blank control group;but there are the tendency that the the average daily gain of terramycin group lower 0.67% than the control one;there were no significant difference among all the 8 groups(P > 0.05) on the average daily gain;The effect of the two chitosans on mice in pilot stage intake and daily gain was not significant (P > 0.05).The effects of intake and daily gain by different adding level on mice were not significant (P intake 1 = 0.407,P intake 2 = 0.087,P ADG 1 = 0.998,P ADG 2 = 0.994,P> 0.05).3. The affection of 1st chitosan on T-lymphocyte better than the 2nd chitosan,but the difference was not significant (P > 0.05);there were significant differences among the three levels of the 1st chitosan(P <0.05),and the medium and highest supplement was extremely remarkable higher than the low dose(P < 0.01);there were terribly significant differences among the three levels of the 2nd chitosan(P <0.01),the T-lymphocyte of the 6st group was extremely higher than the other two doses(P <0.01);the T-lymphocyte percentage of the 3rd,6th groups were higher than the control group(P<0.01)and the terramycin group(P < 0.05).There were no remarkable differences (P> 0.05)on body weight/(liver+spleen)of each group; except the 4th group,the K value of other groups were obviously higher than the control one.The 1st chitosan on the impact of phagocytic index higher than the 2nd ,but the difference was not significant (P > 0.05);the different levels on phagocytic index of both of chitosans were not significantly (P>0.05).The blood serum bacteriolysin content of each group were terribly higher than the control group(P<0.01);both of chitosans on serum activity of lysozyme were increased significantly (P1 = 0.000,P2 = 0.000)with the additive amont increasing,and the 1st one was significantly higher than the 2nd one(P<0.05),The protection rate of infectious individuals of control group and the terramycin group were 50%,70% respectively;the protection rate of infectious individuals of the 1st chitosan were 60%,70% and 70%,while the 2nd one were 50%,50%,60%.The result suggested that both of chitosans played protective role and the function increased with the chitosan's amount increasing.