Different Agronomic Measures To Alleviate The Consecutive Monoculture Problem Of Rehmannia Glutinosa Libosch

Rehmannia glutinosa Libosch. is a perennial herb of Scrophulariaceaea,which is famous and used frequently in China. The Rehmannia medicine has a longhistory of clinical efficacy. It is an important part of the modern medicine. However,the land of Rehmannia glutinosa cultivation must be replanted after a period of8-10years, because of so called “relanting desease”, which severely restricts theproduction of Rehmannia glutinosa. Thus it has become an urgent problem to relieveand eliminate the consecutive monoculture problem of Rehmannia glutinosaLibosch.．In this work, the residue substrate of edible mushroom was added to thecontinuous cropping soil to degrade phenolic acids with aims to relieve the effect ofcontinuous cropping obstacle. Meanwhile, the work explored the mitigation of theconsecutive monoculture problem by mixing the first cropping soil of Rehmanniaglutinosa Libosch.. The aim of work was to provide a theoretical basis for theremission consecutive monoculture problem of Rehmannia glutinosa Libosch. Themain results were as follows:(1) In this study, phenolic acids (hydroxybenzoic acid, vanillic acid, eugenol,vanillin and ferulic acid) were degraded by the extractions of all the tested residuemushroom substrate (SMS) and the maximal degradation rate was75.3%, contributedby extraction of SMS of Pleurotus eryngii Quel. The next was the extraction ofPleurotus nebrodensis, Pleurotus ostreatus and Pleurotus cornucopiae, then Lentinusedodes, Hericium erinaceus and Flammulina velutipes. The worst was the extractionof Auricularia auricula. So the extraction of SMS of Pleurotus eryngii Quel had themaximal potential for the degradation of phenolic acids in soil(2) Pot experiment indicated that hydroxybenzoic acid and vanillin in soil werealso degraded effectively by SMS of Pleurotus eryngii Quel, and their degradation rates were92.5%and100%. The further study showed that the application of residuesubstrate of edible mushroom could make the indicators substantially equivalent tothe level of the first cropping of Rehmannia glutinosa Libosch, such as the crownwidth, number of leaves, leaf length, leaf width,height and so on. In addition, thecropping Rehmannia root fresh weight and dry weight were increased by2.70and3.66times and improved the total plant catalpol2.25times. Furthermore, the numberof ground rhizosphere soil bacteria, fungi and actinomycetes were added, the enzymeactivities of rhizosphere soil were increased, such as invertase, cellulase, urease,phosphatase and peroxidation catalase. These results showed the consecutivemonoculture problems of Rehmannia glutinosa Libosch could be alleviated and therhizosphere soil microenvironment could be improved by the residue substrate ofedible mushroom.(3) Pot experiment showed that the first cropping and1year cropping plantingsoil were mixed in different proportions of Rehmannia glutinosa Libosch had verydifferent results. The disorder symptoms performed significantly when the proportionof the1year cropping soil was added, especially the ratio was larger than1:1. Whilethe ratio was3:1had a slight improvement.In some extent, the consecutive monoculture problems of Rehmannia glutinosaLibosch. was alleviated in this study. But the mechanism obstacle of Rehmanniaglutinosa Libosch. still was needed a in-depth further study.