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Under Different Fertilization Broomcorn Millet Leaf Senescence And Activated Oxygen Metabolism Studies

Posted on:2015-07-03Degree:MasterType:Thesis
Country:ChinaCandidate:N LiFull Text:PDF
GTID:2283330434465007Subject:Crops
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In the arid regions of northern China, broomcorn millet is one of the important major grain crops,which is drought-enduring, with a short growth period, playing an indispensable role in the crop structure optimization. With the improvement of modern living standards, and the increase of health consciousness, the development of modern functional foods has become a hot research topic, broomcorn millet has medicinal value, therefore it becomes an important resource for modern functional food development. However, due to the relatively weak research on broomcorn millet, especially the lack of systematic research physiological characteristics of broomcorn millet cultivation, coupled with the lack of scientific management field, the production is difficult to upgrade. Therefore, to explore the internal physiological characteristics of broomcorn millet plants for breeding varieties and efficient cultivation technology research provides theoretical support, and ultimately achieve the purpose of high yield and quality become the top priority of broomcorn millet production.In this study, Ulmus japonica varieties of millet minced2is the material, no fertilizer is the control group, seting high, medium and low levels of fertilizer, plant metabolic processes by Millet grain filling period of maturation in chlorophyll content (SPAD), soluble protein malondialdehyde (MDA), superoxide anion (O2) content and related indicators of superoxide dismutase (SOD), peroxidase (POD) activity, revealing the conditions under different fertilizer levels Millet leaf senescence and changes of active oxygen metabolism, providing a theoretical basis for the cultivation of high yield and quality of the Loess Plateau Millet drought. The study has reached the following conclusions:(1) During the filling of millet grain, with the senescence of plants, the main stem function chlorophyll content (SPAD) and soluble protein contents basically follow the same trend, showing a downward trend. In the main stem of the same fertilization same growth stages of different position leaves are as follows:flag leaf> second leaf> down clover. Under different fertilizer levels, at the same time in the same position leaves reproductive performance:higher chlorophyll content under high fertility levels (SPAD) and soluble protein content, the latter filling has a slower degradation rate. (2) With the senescence of plants, during the filling of reactive oxygen metabolites produced Millet main stem of non-functional leaves a protective effect. After flowering10d, the main stem top clover superoxide dismutase (SOD), peroxidase (POD) activity decreased protective membrane structure was damaged, the cell physiology decreased membrane lipid peroxidation propionate dialdehyde (MDA), superoxide anion (O2) and other substances accumulation. The same growth in the same period of the main stem leaf under different fertilizer levels of SOD and POD showed as follows:flag leaf> second leaf> down clover, MDA, contrary O2accumulation, the performance of flag leaf<second leaf<back three leaves.Under different fertilizer levels, at the same time in the same position leaves reproductive performed as follows:in high fertility levels of SOD, POD activity is higher, namely F3> F2> F1>F0, while the opposite MDA and O2content, the performance of high fertility levels Under the MDA and O2content is low, namely F3<F2<F1<F0.(3) Fertilization has a great impact on plant stem diameter broomcorn millet, grain weight and yield relatively large, in the high-fat conditions, the plants have thicker main stem, larger grain weight, and grain yield was significantly higher than without fertilizer and in low-fat, indicating that fertilization is conducive to robust plant growth, stem stout, plump full of grain filling, grain weight is higher, resulting in higher yields.The comprehensive analysis shows that during the broomcorn millet filling maturation, different fertilization maintain a certain influence on functions for the main stem of the leaf function and metabolism of active oxygen, certain fertility can promote the growth of broomcorn millet plants robust, thick stem, leaves the main stem function protective enzymes SOD, POD activity increased, scavenging reactive oxygen radicals strong ability to mitigate the extent of injury to the cell membrane structure and function, effectively delaying the leaf senescence, the duration of leaf function has prolonged, fully plump grain filling, ultimately, a higher grain yield. Therefore, the production should be based on broomcorn millet proper fertilization of soil fertility, especially increasing the amount of fertilizer in sandy soil, taking effective measures to strengthen field management fertilizer and promote healthy plant growth and delay leaf senescence, thus improving the photosynthetic plants performance, and promote grain filling mature and achieve high yield of the broomcorn millet.
Keywords/Search Tags:Broomcorn millet, Leaves, Senescence, Reactive oxygen metabolism, Physiological characteristics
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