The Study On Utilization Of Prickly Ash Seed In Diet For Jian Carp (Cyprinus Carpio Var.Jian)

With the rapid development of aquaculture industry, the long-term sustainability of fishfarming might be threatened by its present overdependence on fish oil (FO) and soybean oil(SO) due to the emerging tendency of the shortage of FO and SO production. Therefore,looking for new sources partial or total replacement of FO and SO in aquatic feed with animalor plant fat-rich ingredients has become a focus. With the matching equilibrium C18polyunsaturated fatty acid (PUFA), Chinese prickly ash seed (CPAS) is a good alternative toFO and SO for relatively low cost. However CPAS has the disadvantage of easy oxidation ofthe lipid rancidity. This study was going to make efforts on the follow topics:(1) The reseachon nutritional properties and storage features of CPAS after extruded diet;(2) Effect of dietaryprickly ash seed on the growth performance, body composition and health status in juvenileJian carp (Cyprinus carpio var. Jian). The aim of the study was establish the feed system andexplored the long-term storage method of CPAS. And this study was conducted to investigatethe effects of dietary increasing levels of CPAS for Jian carp, by evaluating the effect ofCPAS on growth performance, feed utilization, body composition, biological parameters,serum biochemical indices, composition of fatty acid and intestinal histological analysis, toprovide reference information for the development of nutritional completely formulatedaquafeed of carp and accurately utilizing CPAS seed more efficiently.(1) This study investigated the nutritional properties and storage features of CPAS mixedwith wheat bran and clear flour after extruded diet. The ratio of CPAS is about33.34%,40%,50%,60%,66.67%. Then compared with a batch of raw material nutritional properties ofexpanded and expanded. And extracting the ingredients’ grease, we determined the acid valueand peroxide value. The experiment results showed as follow: The crude protein and crudelipid content increased along with the increased CPAS levels among all the CPAS-basedgroups. But no significant difference of crude protein and crude lipid content were foundamong the treatments. Moreover, the acid value and peroxide value increased along with theincreased CPAS levels among all the groups. But that the acid value of extruded feed groupwas markedly lower than that grinding feed in the50%CAPS of wheat bran and clear flourgroups. What’s more, the peroxide value of extruded feed group was markedly lower thanthat grinding feed in the40%and50%CAPS of wheatbran. And the peroxide value of extruded feed group was significantly lower than that grinding feed only in the60%CAPS ofwheat bran.(2) This study analyzed the effects of different concentrations of CPAS on growthparameters, the proximate body composition and health status in the diet of jian carp(Cyprinus carpio var. Jian). Jian carps were fed one of four diets triple daily to satiation for60days. Jian carps were given diets with incremental concentrations of CPAS (0.0%,2.5%,5.0%, and7.5%) as a replacement of soybean oil (control). The results showed that fish fed5%CPAS obtained highest specific growth rate and lowest with7.5%CPAS. Feed efficiencywas highest at2.5%and lowest at7.5%. No significant differences of moisture, crude protienand total lipids contents were found among fillets from tilapia fed the different diets. Therewere also no adverse effects of the diet treatments on fish health, as determined from analysisof various haematological and superoxide dismutase. The highest vaule of malondialdehydewas accompanied by the diet of7.5%CPAS. Fatty acid compositions of muscle, liver and IPFwere correlated with dietary fatty acids. Fish muscle, liver and IPF concentration of20:5n-3and22:6n-3increased with increasing dietary18:3n-3(n-3HUFA). Normal histologicalstructures in midgut tissues were observed in all groups while there showed a significantlylower folds height and width of fish fed diets supplemented with7.5%.Conclusion:(1)50%CAPS of wheat bran and clear flour have higher grease content. And50%CAPSgroups are more conducive to storage and better application effect.(2) Results of the present study demonstrated that High intakes of dietary7.5%CPASresulted in reduced growth performance and the health status of feed of jian carp. And adding5%CPAS in jian carp diet without compromising growth performance and the health status.