Cloning And Expression Characteristics Of Dynactin P62Gene In The Apis Cerana Cerana

With the A. cerana cerana as materials and the genomic sequences of Apis melliferaas reference in this study,we cloned the genomic DNA sequence and mRNA sequence ofdynactin p62of A. cerana cerana. Meanwhile, quantitative analysis of the expression levelof dynactin p62gene at different developmental stages bees were conducted usingreal-time PCR to explore the methylation characteristic of dynactin p62gene in the Apiscerana cerana. On these basises we fed bee larvaes in experimental groups with differentnutrition and space to explore the expression characteristics of dynactin p62gene in the6-days larvae of Apis cerana cerana.By cloning and sequencing technology, the results show that the full-length genomicDNA sequences (GenBank accession no. JX101463) and mRNA sequences (GenBankaccession no. JX101464) of A. cerana cerana dynactin p62gene are2403bp and1491bp.Phylogenetic analysis indicated that A. c. cerana dynactin p62and A. mellifera dynactinp62gathered in the same clade.Quantitative analysis of the expression level of dynactin p62at differentdevelopmental stages of the queen, worker and drone (3and6d-old larvae, and newlyeclosed bees) were conducted using real-time PCR. The A. c. cerana dynactin p62transcript was clearly detected at different developmental stages of the queens and workers.And it was expressed significantly higher in newly eclosed adults than in larvae (P<0.05),and was higher in workers than in queens (P<0.05), while no obvious regularity existed indrone. These results suggested that dynactin p62may be involved in caste differentiationof female honeybees in A. c. cerana.Through artificial feeding the bee larvaes with different nutrition and in differentspace, then quantitative analysis of its expression level of dynactin p62gene by usingreal-time PCR. The results showed that the A. c. cerana dynactin p62transcript wasdetected at different larvae groups, and it was no significantly expression between thegroup with different nutrition (P<0.05) and expressed significantly between the group indifferent space (P>0.05).