Technical Research On Use Of NaCl For Screening Cabbage Salt Tolerant Mutant In Vitro

Studying cabbage salt-resistance, discussing salt-resistance identification ofsalt-tolerance breeding resource, researching new salt-tolerant varieties and solving theproblems of soil salinization are important subjects full of challenge about agriculturaldevelopment in the future, which have important theoretical and practical significance. In thisexperiment, firstly, choosing the Qingan80, the Yuexiawang and the F52as test materials,which are hybrids of different salt-resistance, the different salt concentration are setted toresearch cabbage salt resistance in seed germination stage and in seedling stage, and acabbage salt resistance identification method is gained. Secondly, choosing DH12-7, DH136and DH149as materials, the different salt concentration and treat time are setted up to screenin vitro cells of microspore by microspore culture and the callus for regeneration cabbageplants by leaf regeneration, and a method of screening salt tolerant mutants are received toscreen cabbage salt tolerant mutant plants. Finally, selecting the cabbage microspore haploidplants H10-23, H10-3, H10-5as test materials, different colchicine treatment concentrationand processing time are setted to discuss the way to double by treating blades and callus incolchicine. In this study the main results are as follows：1. NaCl salt stress inhibits cabbage seed germination and seedlings growth, and cause thehappening of the salt damage. Result of salt resistance identification in seed germinationperiod and that in seedling stage are consistent. Salt solution method with use of0.9%NaCl,the optimal salt concentration for the salt resistance identification in seedling stage and seedgermination stage of cabbage, is the optimal method for salt resistance appraisal.2. The optimal selection concentration in the microspore culture is200mg/L NaCl for itstotal number of regeneration plants is9, and its survival rate of regeneration plants is ashighest as14.75%.3. Choosing NaCl as a salt selection pressure of screening salt tolerant mutant in vitro, itsconcentration and processing time effect the result of screening salt-resistant mutants in vitro.In summary, the method of in vitro screening salt resistant variants is to screen callus in vitrofor3weeks in300mmol/L salt concentration after UV mutagenesis for3min, then to screen repeatedly, and finally to obtain the salt-resistant variant plants by the salt-resistanceidentification. In this study7,17and13salt-tolerant mutant plants of DH12-7, DH131, andDH149are obtained preliminarily.4. Average double rate of DH plants is58.13%by putting callus from cabbagemicrospore haploid plants’ leaf in colchicine of2.0mg/L for30h.