Sequence Analysis And Polymorphism Study Of The CYGB Gene Intron In Yak

In order to study the yak CYGB gene sequence information, the full sequence of CYGB geneof yak was cloned. The sequence structure, composition of nucleotide, and the basic physical andchemical properties, senior structure were analyzed by using Lasergene7, PredictProtein,SWISS-MODEL and other biological software related. While analysising the polymorphisms inintron sequence of CYGB gene in300samples of Gannan yak and283samples of Gansu hexisimmental by PCR-SSCP.1. The CYGB gene sequence in yak was amplified with cloning and bioinformaticsanalysis shows: The full DNA sequence was8484bp in length, including4exons and3introns.The length of4exons was143bp,232bp,164bp and34bp respectively, the length of3intronswas5175bp,280bp and2379bp respectively. The CDS was573bp. The G+C%(60.32%) higherthan A+T%(39.68%) in the full sequence. It was predicted that yak CYGB was a kind of solubleprotein with190amino acids sequence, and the molecular weight was about21399.6D,isoelectric point was about6.32. The secondary structure was mainly α-helix（60.53%）andloop（39.47%）, CYGB was all the alpha class protein. Phylogenetic analysis suggests that theCYGB gene molecular evolution tree and species tree was similar on structure, phylogeneticanalysis showed that homology was above93%among Bos taurus、Ovis aries and other species.The deduced encoded product was located in the cytoplasm, may play an important role of signaltransduction, transcriptional regulation in the process of energy metabolism and biosynthesis ofcofactors.2. The polymorphisms of three introns of CYGB gene were detected. Results are as follows:181bp sequence of intron1that was amplified by the P5primer was controlled by A, B, C allelesin Gannan yak, and formed3genotypes, named AA, AB and AC, and there was only onegenotype named DD and not polymorphism in Simmental.319bp sequence of intron2that wasamplified by the P6primer was controlled by A, B, C and D alleles in Gannan yak, and formed4genotypes, named AA, AB AC and AD, and there was2genotypes in Simmental, named EE andEF, controlled by E and F alleles.300bp sequence of intron3that was amplified by the P7primer was only controlled by A and B alleles in Gannan yak, and formed2genotypes, namedAA and AB, and there was3genotypes in Simmental, named CC、CD and CE, controlled by C,D and E alleles.